Novel nanomaterial-based pesticide formulations are increasingly perceived as promising aids in the transition to more efficient agricultural production systems. The current understanding of potential unintended (eco)toxicological impacts of nano-formulated pesticides is scarce, in particular with regard to (non-target) aquatic organisms and ecosystems. The present study reports the results of a long-term freshwater mesocosm experiment which assessed responses of individual zooplankton taxa and communities to a novel TiO₂-coated nano-formulation of the fungicide carbendazim. Population- and community trends were assessed and compared in response to the nano-formulation and its constituents applied individually (i.e. nano-sized TiO₂, carbendazim) and in combination (i.e. nano-sized TiO₂ & carbendazim). Minimal differences were observed between effects induced by the nano-formulation and its active ingredient (i.e. carbendazim) when applied at equivalent nominal test concentrations (4 μg L⁻¹). Nano-sized TiO₂ was found to affect zooplankton community trends when applied separately at environmentally realistic concentrations (20 μg L⁻¹ nominal test concentration). However, when nano-sized TiO₂ was applied in combination with carbendazim, nano-sized TiO₂ was found not to alter effects on community trends induced by carbendazim. The findings of the current study provide an extensive and timely addition to the current body of work available on non-target impacts of nano-formulated pesticides.

基于纳米材料的新型农药制剂越来越被认为是向更高效的农业生产系统过渡的有希望的帮助。目前对纳米农药潜在的意外（生态）毒理学影响的了解还很少，特别是对于（非目标）水生生物和生态系统。本研究报告了一项长期淡水中生态实验的结果，该实验评估了单个浮游动物类群和群落对新型 TiO ₂涂层杀菌剂多菌灵纳米制剂的反应。_{根据单独应用（即纳米TiO 2}、多菌灵）和组合应用（即纳米TiO ₂和多菌灵）的纳米制剂及其成分，评估和比较了种群和群落趋势。^{当以等效标称测试浓度（4 μg L -1}）施用时，纳米制剂及其活性成分（即多菌灵）引起的效果之间观察到最小差异。^{当以环境实际浓度（20 μg L -1}标称测试浓度）单独施用时，发现纳米级TiO ₂会影响浮游动物群落趋势。然而，当纳米TiO ₂与多菌灵结合使用时，发现纳米TiO _{2不会改变多菌灵引起的群落趋势的影响。}当前研究的结果为当前有关纳米农药非目标影响的工作提供了广泛而及时的补充。