VqMAPK3/VqMAPK6, VqWRKY33, and VqNSTS3 consist of a regulatory node in enhancing resistance to powdery mildew in grapevine,Horticulture Research

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VqMAPK3/VqMAPK6, VqWRKY33, and VqNSTS3 consist of a regulatory node in enhancing resistance to powdery mildew in grapevine
Horticulture Research ( IF 7.6 ) Pub Date : 2023-05-31 , DOI: 10.1093/hr/uhad116
Wandi Liu _{1,

2,

3} , Chaohui Yan _{1,

2,

3} , Ruimin Li _{1,

2,

3} , Guanyu Chen _{1,

2,

3} , Xinqi Wang _{1,

2,

3} , Yingqiang Wen _{1,

2,

3} , Chaohong Zhang _{1,

2,

3} , Xiping Wang _{1,

2,

3} , Yan Xu _{1,

2,

3} , Yuejin Wang _{1,

2,

3}

Affiliation

Grapevine powdery mildew is caused by Erysiphe necator that seriously harms grape production in the world. Stilbene synthase makes phytoalexins that contribute to the resistance of grapevine against powdery mildew. A novel VqNSTS3 was identified and cloned from Chinese wild Vitis quinquangularis accession Danfeng-2. Novel VqNSTS3 was transferred into susceptible ‘Thompson Seedless’ by Agrobacterium-mediated transformation. The transgenic plants showed resistance to the disease and activated other resistance-related genes. VqNSTS3 expression in grapevine is regulated by VqWRKY33, which binds to TTGACC in the VqNSTS3 promoter with fungal elicitor responsive element. Furthermore, VqWRKY33 was phosphorylated by VqMAPK3/VqMAPK6 and thus lead to enhanced signal transduction and increased VqNSTS3 expression. ProVqNSTS3::VqNSTS3-GFP of transgenic VqNSTS3 in Arabidopsis thaliana was observed to move to and wrap the pathogen haustoria and block the invasion of Golovinomyces cichoracearum. These results demonstrated that stilbene accumulation of novel VqNSTS3 of a Chinese wild grapevine prevented pathogen invasion and enhanced resistance to powdery mildew. Therefore, VqNSTS3 can be used in generating powdery mildew-resistant grapevines.

更新日期：2023-05-31

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