Herein, a Faraday cage-type electrochemiluminescence biosensor was designed for the detection of human breast cancer cell MCF-7. Two kinds of nanomaterials, Fe₃O₄-APTs and GO@PTCA-APTs, were synthesized as capture unit and signal unit, respectively. In presence of the target MCF-7, the Faraday cage-type electrochemiluminescence biosensor was constructed by forming a complex “capture unit-MCF-7-signal unit”. In this case, lots of electrochemiluminescence signal probes were assembled and could participate in the electrode reaction, achieving a significant increase in sensitivity. In addition, the double aptamer recognition strategy was adopted to improve the capture, enrichment efficiency and detection reliability. Under optimal experimental conditions, the limit of detection was 3 cells/mL. And, the sensor could afford the detection of actual human blood samples, which is the first report on the detection of intact circulating tumor cells by the Faraday cage-type electrochemiluminescence biosensor.

本文设计了法拉第笼型电化学发光生物传感器用于检测人乳腺癌细胞MCF-7。合成了两种纳米材料Fe ₃ O ₄ -APTs和GO@PTCA-APTs，分别作为捕获单元和信号单元。在目标MCF-7存在的情况下，通过形成复杂的“捕获单元-MCF-7-信号单元”构建了法拉第笼型电化学发光生物传感器。在这种情况下，大量的电化学发光信号探针被组装起来并可以参与电极反应，实现灵敏度的显着提高。此外，采用双适配体识别策略，提高捕获、富集效率和检测可靠性。在最佳实验条件下，检测限为3个细胞/mL。并且，该传感器可以对实际的人体血液样本进行检测，这是首次利用法拉第笼式电化学发光生物传感器检测完整的循环肿瘤细胞的报道。