Due to the excellent oxidation effect on phenolic compounds, the present copper-based laccase-mimics are mostly used for the degradation and detection of phenolic pollutants in environment. As the oxidation substrates of laccase are only limited to compounds containing specific phenolic hydroxyl groups, which makes the application of laccase mimetics to non-phenolic pollutants faces great challenges and bottlenecks. Herein, a non-copper-based laccase mimics like cubic Ag₂O nanoparticles is prepared. The maximum reaction velocity and affinity of cubic Ag₂O nanoparticles to substrates are significantly better than that of natural laccase and most artificial laccase mimetics. Cubic Ag₂O nanoparticles also exhibits high stability under various conditions and has good tolerance to organic solvents. Moreover, it is found that the laccase-mimic activity of cubic Ag₂O nanoparticles can be effectively inhibited by kanamycin, which enable us to construct a colorimetric sensor for rapid detection of kanamycin in environment. The sensor has excellent sensitivity with the limit of detection (LOD) of 1.3 nM for kanamycin, and has high selectivity for other competitive substances. Besides, a standard curve for rapidly detecting kanamycin by smartphones is established, which expect to provide a novel idea for on-site monitoring of other hazards in environment.

由于对酚类化合物具有优异的氧化作用，目前铜基漆酶模拟物主要用于环境中酚类污染物的降解和检测。由于漆酶的氧化底物仅限于含有特定酚羟基的化合物，这使得漆酶模拟物在非酚类污染物中的应用面临着巨大的挑战和瓶颈。在此，制备了非铜基漆酶模拟物，如立方 Ag _{2 O 纳米粒子。立方Ag 2} O纳米粒子的最大反应速度和对底物的亲和力明显优于天然漆酶和大多数人工漆酶模拟物。立方银₂O 纳米粒子在各种条件下也表现出高稳定性，并且对有机溶剂具有良好的耐受性。此外，发现立方Ag ₂ O纳米粒子的漆酶模拟活性可以被卡那霉素有效抑制，这使我们能够构建用于快速检测环境中卡那霉素的比色传感器。该传感器具有优异的灵敏度，卡那霉素的检测限（LOD）为1.3 nM，对其他竞争物质具有高选择性。此外，建立了智能手机快速检测卡那霉素的标准曲线，有望为环境中其他危害的现场监测提供新思路。