Advances in deoxyribonucleic acid extraction techniques and point-of-care molecular diagnosis of foodborne pathogens,Analyst

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Advances in deoxyribonucleic acid extraction techniques and point-of-care molecular diagnosis of foodborne pathogens
Analyst ( IF 3.6 ) Pub Date : 2023-05-31 , DOI: 10.1039/d3an00045a
Rajamanickam Sivakumar ₁ , Nae Yoon Lee ₁

Affiliation

A conventional molecular assay-based point-of-care (POC) diagnostic test involves three major stages: deoxyribonucleic acid (DNA) extraction, amplification, and amplicon detection. Among these steps, DNA extraction is costly and time-consuming. Nevertheless, it is a crucial step for the identification of sensitive and specific diseases. This review summarizes the advantages and disadvantages of DNA extraction methods over the past 10 years to effectively implement POC pathogen testing in the future. The first section briefly explains the necessity of DNA extraction and molecular assays for food pathogen detection. The second section extensively discusses DNA extraction based on liquid–liquid extraction, solid-phase extraction, and electrophoretic techniques. Molecular assay-based methods and a few commercially available POC devices for the detection of foodborne pathogens are detailed in the third and fourth sections. Finally, present challenges and future perspectives for the fabrication of integrated POC devices are highlighted.

中文翻译：

脱氧核糖核酸提取技术和食源性病原体即时分子诊断的进展

传统的基于分子测定的即时护理 (POC) 诊断测试涉及三个主要阶段：脱氧核糖核酸 (DNA) 提取、扩增和扩增子检测。在这些步骤中，DNA提取既昂贵又耗时。尽管如此，这是识别敏感和特定疾病的关键一步。本综述总结了过去10年DNA提取方法的优缺点，以期在未来有效实施POC病原体检测。第一部分简要阐述了 DNA 提取和分子检测在食品病原体检测中的必要性。第二部分广泛讨论基于液液萃取、固相萃取和电泳技术的 DNA 提取。第三和第四部分详细介绍了基于分子测定的方法和一些市售的 POC 设备，用于检测食源性病原体。最后，强调了集成 POC 器件制造目前面临的挑战和未来前景。

更新日期：2023-05-31

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