Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Silicon chambers for enhanced incubation and imaging of microfluidic droplets
Lab on a Chip ( IF 6.1 ) Pub Date : 2023-05-31 , DOI: 10.1039/d2lc01143c Nicolas Lobato-Dauzier 1, 2 , Robin Deteix 1, 2 , Guillaume Gines 3 , Alexandre Baccouche 1, 2 , Benediktus Nixon Hapsianto 2 , Shu Okumura 1, 2 , Guilhem Mariette 1, 2 , Djaffar Belharet 4 , Samuel Queste 4 , Laurent Jalabert 1, 2 , Matthieu Denoual 5 , Yannick Rondelez 3 , Hiroshi Toshiyoshi 1, 2 , Hiroyuki Fujita 6 , Soo Hyeon Kim 1, 2 , Teruo Fujii 1, 2 , Anthony J Genot 1, 2
Lab on a Chip ( IF 6.1 ) Pub Date : 2023-05-31 , DOI: 10.1039/d2lc01143c Nicolas Lobato-Dauzier 1, 2 , Robin Deteix 1, 2 , Guillaume Gines 3 , Alexandre Baccouche 1, 2 , Benediktus Nixon Hapsianto 2 , Shu Okumura 1, 2 , Guilhem Mariette 1, 2 , Djaffar Belharet 4 , Samuel Queste 4 , Laurent Jalabert 1, 2 , Matthieu Denoual 5 , Yannick Rondelez 3 , Hiroshi Toshiyoshi 1, 2 , Hiroyuki Fujita 6 , Soo Hyeon Kim 1, 2 , Teruo Fujii 1, 2 , Anthony J Genot 1, 2
Affiliation
|
Droplet microfluidics has become a powerful tool in life sciences, underlying digital assays, single-cell sequencing or directed evolution, and it is making foray in physical sciences as well. Imaging and incubation of droplets are crucial, yet they are encumbered by the poor optical, thermal and mechanical properties of PDMS, a material commonly used in microfluidics labs. Here we show that Si is an ideal material for droplet chambers. Si chambers pack droplets in a crystalline and immobile monolayer, are immune to evaporation or sagging, boost the number of collected photons, and tightly control the temperature field sensed by droplets. We use the mechanical and optical benefits of Si chambers to image ≈1 million of droplets from a multiplexed digital assay – with an acquisition rate similar to the best in-line methods. Lastly, we demonstrate their applicability with a demanding assay that maps the thermal dependence of Michaelis–Menten constants with an array of ≈150 000 droplets. The design of the Si chambers is streamlined to avoid complicated fabrication and improve reproducibility, which makes Si a complementary material to PDMS in the toolbox of droplet microfluidics.
中文翻译：
用于增强微流体液滴的孵育和成像的硅室
液滴微流体已成为生命科学、基础数字化验、单细胞测序或定向进化的强大工具，它也在物理科学领域取得进展。液滴的成像和孵育至关重要，但它们受到 PDMS 较差的光学、热学和机械性能的阻碍，PDMS 是微流体实验室常用的一种材料。在这里，我们表明 Si 是液滴室的理想材料。Si 室将液滴包裹在结晶且固定的单层中，不受蒸发或下垂的影响，增加收集的光子数量，并严格控制液滴感应到的温度场。我们利用 Si 腔室的机械和光学优势对来自多路复用数字化验的大约 100 万个液滴进行成像——采集率与最佳在线方法相似。最后，我们通过苛刻的分析证明了它们的适用性，该分析映射了 Michaelis-Menten 常数与约 150 000 个液滴阵列的热依赖性。Si 腔室的设计经过流线化以避免复杂的制造并提高可重复性，这使得 Si 成为液滴微流体工具箱中 PDMS 的补充材料。
更新日期：2023-05-31
中文翻译：
用于增强微流体液滴的孵育和成像的硅室
液滴微流体已成为生命科学、基础数字化验、单细胞测序或定向进化的强大工具，它也在物理科学领域取得进展。液滴的成像和孵育至关重要，但它们受到 PDMS 较差的光学、热学和机械性能的阻碍，PDMS 是微流体实验室常用的一种材料。在这里，我们表明 Si 是液滴室的理想材料。Si 室将液滴包裹在结晶且固定的单层中，不受蒸发或下垂的影响，增加收集的光子数量，并严格控制液滴感应到的温度场。我们利用 Si 腔室的机械和光学优势对来自多路复用数字化验的大约 100 万个液滴进行成像——采集率与最佳在线方法相似。最后，我们通过苛刻的分析证明了它们的适用性，该分析映射了 Michaelis-Menten 常数与约 150 000 个液滴阵列的热依赖性。Si 腔室的设计经过流线化以避免复杂的制造并提高可重复性，这使得 Si 成为液滴微流体工具箱中 PDMS 的补充材料。
京公网安备 11010802027423号