In the context of continued spread of coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 and the emergence of new variants, the demand for rapid, accurate, and frequent detection is increasing. Moreover, the new predominant strain, Omicron variant, manifests more similar clinical features to those of other common respiratory infections. The concurrent detection of multiple potential pathogens helps distinguish SARS-CoV-2 infection from other diseases with overlapping symptoms, which is significant for providing tailored treatment to patients and containing the outbreak. Here, we report a lab-on-a-chip biosensing platform for SARS-CoV-2 detection based on the subwavelength grating micro-ring resonator. The sensing surface is functionalized by specific antibody against SARS-CoV-2 spike protein, which could produce redshifts of resonant peaks by antigen–antibody combination, thus achieving quantitative detection. Additionally, the sensor chip is integrated with a microfluidic chip featuring an anti-backflow Y-shaped structure that enables the concurrent detection of two analytes. In this study, we realized the detection and differentiation of COVID-19 and influenza A H1N1. Experimental results indicate that the limit of detection of our device reaches 100 fg/ml (1.31 fM) within 15 min detecting time, and cross-reactivity tests manifest the specificity of the optical diagnostic assay. Furthermore, the integrated packaging and streamlined workflow facilitate its use for clinical applications. Thus, the biosensing platform presents a promising approach for attaining highly sensitive, selective, multiplexed, and quantitative point-of-care diagnosis and distinction between COVID-19 and influenza.

中文翻译：

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使用亚波长光栅微环谐振器快速检测和区分 COVID-19 病毒 (SARS-CoV-2) 和流感病毒的护理点生物传感器

在由 SARS-CoV-2 引起的 2019 冠状病毒病（COVID-19）持续传播和新变种出现的背景下，对快速、准确和频繁检测的需求日益增加。此外，新的主要菌株 Omicron 变种表现出与其他常见呼吸道感染更相似的临床特征。同时检测多种潜在病原体有助于将 SARS-CoV-2 感染与其他具有重叠症状的疾病区分开来，这对于为患者提供量身定制的治疗和遏制疫情爆发具有重要意义。在这里，我们报告了一种基于亚波长光栅微环谐振器的用于 SARS-CoV-2 检测的芯片实验室生物传感平台。传感表面通过针对SARS-CoV-2刺突蛋白的特异性抗体进行功能化，可以通过抗原抗体结合产生共振峰红移，从而实现定量检测。此外，传感器芯片与具有防回流Y形结构的微流控芯片集成，可以同时检测两种分析物。在本研究中，我们实现了COVID-19和甲型H1N1流感的检测和区分。实验结果表明，我们的装置的检测限在 15 分钟检测时间内达到 100 fg/ml (1.31 fM)，交叉反应测试证明了光学诊断测定的特异性。此外，集成的包装和简化的工作流程有利于其在临床应用中的使用。因此，生物传感平台提供了一种有前途的方法，可实现高度灵敏、选择性、多重和定量的护理点诊断以及区分 COVID-19 和流感。