Discovery of Peptidic Ligands against the SARS-CoV-2 Spike Protein and Their Use in the Development of a Highly Sensitive Personal Use Colorimetric COVID-19 Biosensor,ACS Sensors

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Discovery of Peptidic Ligands against the SARS-CoV-2 Spike Protein and Their Use in the Development of a Highly Sensitive Personal Use Colorimetric COVID-19 Biosensor
ACS Sensors ( IF 8.2 ) Pub Date : 2023-05-30 , DOI: 10.1021/acssensors.2c02386
Xingjian Yu _{1,

2} , Bofeng Pan ₃ , Cunyi Zhao ₃ , Diedra Shorty _{1,

2} , Lucas N Solano ₁ , Gang Sun ₃ , Ruiwu Liu ₁ , Kit S Lam ₁

Affiliation

In addition to efficacious vaccines and antiviral therapeutics, reliable and flexible in-home personal use diagnostics for the detection of viral antigens are needed for effective control of the COVID-19 pandemic. Despite the approval of several PCR-based and affinity-based in-home COVID-19 testing kits, many of them suffer from problems such as a high false-negative rate, long waiting time, and short storage period. Using the enabling one-bead-one-compound (OBOC) combinatorial technology, several peptidic ligands with a nanomolar binding affinity toward the SARS-CoV-2 spike protein (S-protein) were successfully discovered. Taking advantage of the high surface area of porous nanofibers, immobilization of these ligands on nanofibrous membranes allows the development of personal use sensors that can achieve low nanomolar sensitivity in the detection of the S-protein in saliva. This simple biosensor employing naked-eye reading exhibits detection sensitivity comparable to some of the current FDA-approved home detection kits. Furthermore, the ligand used in the biosensor was found to detect the S-protein derived from both the original strain and the Delta variant. The workflow reported here may enable us to rapidly respond to the development of home-based biosensors against future viral outbreaks.

中文翻译：

针对 SARS-CoV-2 刺突蛋白的肽配体的发现及其在开发高灵敏度个人用比色 COVID-19 生物传感器中的用途

除了有效的疫苗和抗病毒疗法之外，还需要可靠且灵活的家庭个人使用诊断来检测病毒抗原，以有效控制 COVID-19 大流行。尽管多种基于PCR和亲和力的家用COVID-19检测试剂盒已获得批准，但其中许多都存在假阴性率高、等待时间长和保存期短等问题。利用一珠一化合物 (OBOC) 组合技术，成功发现了几种对 SARS-CoV-2 刺突蛋白（S 蛋白）具有纳摩尔级结合亲和力的肽配体。利用多孔纳米纤维的高表面积，将这些配体固定在纳米纤维膜上，可以开发个人使用的传感器，在检测唾液中的 S 蛋白时实现低纳摩尔灵敏度。这种简单的生物传感器采用肉眼读取，其检测灵敏度可与目前 FDA 批准的一些家用检测套件相媲美。此外，生物传感器中使用的配体被发现可以检测源自原始菌株和Delta变体的S蛋白。这里报告的工作流程可能使我们能够快速响应针对未来病毒爆发的家用生物传感器的开发。

更新日期：2023-05-30

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