Vaporized hydrogen peroxide (VHP) sterilization has been widely used in the fields of medical care and pharmaceuticals. Extracellular polymeric substances (EPS) connected tightly to the cell membrane is first barrier that must be broken through, and less is known about direct change of cell membrane peroxides, and the linkage mechanism among EPS, cell membrane and intracellular damages is still unclear during high concentration VHP sterilization. After optimizing the VHP sterilization process of specific drug-packaging equipment based on the sterility assurance level of 10⁻⁶ cfu, we studied the degradation kinetics of exopolysaccharide, exoprotein, extracellular DNA, and investigated the changes of cell membrane lipid/protein peroxides and intracellular peroxidases, and discussed the linkage mechanism of microbial EPS, cell membrane and intracellular damages during high concentration VHP sterilization. The results indicated: (1) The order of degradation kinetics of microbial EPS was exopolysaccharide > exoprotein > extracellular DNA, it was the key to achieve fast and effective VHP sterilization that sufficient high concentration VHP (>400 ppm) was adopted to rapidly destroy EPS (>20 min), resulting in the serious destruction of proteins and lipids of cell membrane; (2) The key mechanism of short time sterilization using high concentration VHP was that the serious loss of the structure/function of cell membrane was the most fatal but the damage of intracellular enzymes and DNA was limited for bacterial cell living.

汽化过氧化氢（VHP）灭菌已广泛应用于医疗和制药领域。与细胞膜紧密相连的细胞外聚合物（EPS）是必须突破的第一道屏障，而细胞膜过氧化物的直接变化知之甚少，EPS、细胞膜和细胞内损伤之间的联动机制尚不清楚。浓度 VHP 灭菌。基于10 ^{−6的无菌保证水平对特定药品包装设备的VHP灭菌工艺进行优化后} cfu，我们研究了胞外多糖、胞外蛋白、胞外DNA的降解动力学，考察了细胞膜脂质/蛋白过氧化物和胞内过氧化物酶的变化，探讨了高浓度VHP灭菌过程中微生物EPS、细胞膜和胞内损伤的联动机制。结果表明：（1）微生物EPS的降解动力学顺序为胞外多糖>胞外蛋白>胞外DNA，采用足够高浓度的VHP（>400 ppm）快速破坏EPS是实现VHP快速有效灭菌的关键(>20 min)，导致细胞膜蛋白质和脂质的严重破坏；