The mitochondrial targeting and viscosity-sensitive near-infrared fluorescence probe TQS-OH was an aggregation induced emission luminogen constructed by regulating the receptor structure and hydroxylating its terminal. TQS-OH presented more than 27.35-fold fluorescence enhancement at the maximum emission peak when the viscosity level increased from 0.49 to 1048 cP. It also appeared excellent reactive oxygen species production ability under white light irradiation and appeared strong photodynamic therapied effect on HepG2 cells. In addition, cellular co-location analysis implied that TQS-OH had accurate mitochondrial targeting ability, with co-location coefficient up to 0.95 in human hepatocellular carcinoma HepG2 cells and it also successfully achieved fatty liver imaging. Meanwhile, we found that TQS-OH induced cancer cell apoptosis by activating mitochondrial apoptosis pathway. Furthermore, TQS-OH ultimately has implemented the tumor photodynamic therapy with high efficiency in vivo, with a tumor inhibition rate as high as 50.77%. The above results made it a promising aggregation induced emission material for the diagnosis of fatty liver and tumor phototherapy.

线粒体靶向粘敏近红外荧光探针TQS-OH是一种通过调节受体结构和末端羟基化而构建的聚集诱导发光体。当粘度水平从 0.49 增加到 1048 cP 时， TQS-OH在最大发射峰处呈现超过 27.35 倍的荧光增强。在白光照射下也表现出优异的活性氧生成能力，对HepG2细胞表现出较强的光动力治疗作用。此外，细胞共定位分析表明TQS-OH具有准确的线粒体靶向能力，在人肝癌HepG2细胞中的共定位系数高达0.95，并成功实现了脂肪肝成像。同时，我们发现TQS-OH通过激活线粒体凋亡通路诱导癌细胞凋亡。此外，TQS-OH最终在体内实现了高效的肿瘤光动力治疗，抑瘤率高达50.77%。上述结果使其成为一种很有前途的聚集诱导发光材料，可用于脂肪肝的诊断和肿瘤的光疗。