The rapid and accurate measurement of Raman signals on the test line (T-line) of surface-enhanced Raman scattering immunochromatography assay (SERS-ICA) strip is necessary for on-site detection based on SERS. Here, a novel linear spot Raman detector which can directly collect the SERS signals of the whole T-line on the dual-diagnostic SERS-ICA strip is developed for detection of multiple respiratory viruses via two magnetic SERS tags within 26 min. The tags modified with two Raman reporter molecules can specifically recognize two viruses, thus enabling the simultaneous detection of both viruses on one T-line. Measured by the linear Raman detector, the limits of detection of the proposed dual-diagnostic SERS-ICA strip is as low as 870 and 263 copies/mL for severe acute respiratory syndrome coronavirus 2 and H1N1, respectively. The dual-diagnostic strip combined with the novel Raman detector herein are of great importance in the differential diagnosis of respiratory pathogens and show potential in the rapid and accurate diagnosis and control of emerging acute respiratory diseases.

表面增强拉曼散射免疫层析法（SERS-ICA）试纸条测试线（T线）上拉曼信号的快速准确测量是基于SERS的现场检测所必需的。在这里，开发了一种新型线性点拉曼检测器，可以在双诊断 SERS-ICA 条上直接收集整个 T 线的 SERS 信号，用于在 26 分钟内通过两个磁性 SERS 标签检测多种呼吸道病毒。用两个拉曼报告分子修饰的标签可以特异性识别两种病毒，从而可以在一条T线上同时检测两种病毒。通过线性拉曼检测器测量，所提出的双重诊断 SERS-ICA 试纸条对严重急性呼吸系统综合症冠状病毒 2 和 H1N1 的检测限分别低至 870 和 263 拷贝/mL。