Filamentous algae from the genus Tribonema presents a challenge in commercial applications because of their slow growth and low oil content. Magneto-electric composite fields (MECs) intervention has been used to increase the development of several plants; however, its effects on algal metabolism are unclear. Here, the Tribonema sp. was cultured under the interventions of ME ($24/d$-$0.6V/cm$, $1h/d$-$0.6V/cm$, $24h/d$-$1.2V/cm$, $1h/d$-$1.2V/cm$) as well as individual MF or EF and control conditions. The objectives are to provide a comparative analysis of individual MF or EF and their synergistic (coupling) effects on biomass accumulation and conversion to fatty acids. The maximum increase in biomass (21.54%), carotenoid (15.9%), carbohydrate (23.1%), and lipids (4.5%) were obtained at $1h/d$-$0.6V/cm$. The productivities (mgL⁻¹d⁻¹) of biomass, carbohydrate, and lipids were increased by 31%, 55%, and 47%, respectively, compared to their controls. For $1h/d$-$1.2V/cm$ intervention, palmitic acid and palmitoleic acid showed an increase of 24.5% and 45.9%, respectively. Furthermore, fatty acids showed improved biodiesel characteristics, including long-chain saturated factor and cold filter plugging point. Genes encoding glycolytic enzymes: Hexokinase, Triose phosphate isomerase, 6-phosphofructokinase, Pyruvate dehydrogenase upregulated or $1h/d$-$.2V/cm\text{.}$ Fatty acid biosynthetic enzymes: Fatty acid synthase, Acyl-carrier-protein - S-malonyltransferase, 3-oxoacyl-ACP-synthase II, 3-oxoacyl-ACP reductase, Enoyl- ACP reductase I, upregulated uunder $1h/d$-$0.6V/cm$, together with downregulation of fatty acids degradation genes. This study, for the first time, provided MECs as an efficient stimulus toward the conversion of carbohydrates into fatty acids in Tribonema for the production of eco-friendly and cost-effective energy.

Tribonema属的丝状藻类因其生长缓慢和含油量低而在商业应用中提出了挑战。磁电复合场 (MECs) 干预已被用于促进多种植物的发展；然而，它对藻类代谢的影响尚不清楚。在这里，Tribonema sp。在ME的干预下进行培养（$24/d$-$0.6V/C米$,$\mathrm{1个}H/d$-$0.6V/C米$,$24H/d$-$1.2V/C米$,$\mathrm{1个}H/d$-$1.2V/C米$) 以及单独的 MF 或 EF 和控制条件。目标是对单个 MF 或 EF 及其对生物量积累和转化为脂肪酸的协同（偶联）效应进行比较分析。生物量 (21.54%)、类胡萝卜素 (15.9%)、碳水化合物 (23.1%) 和脂质 (4.5%) 的最大增加出现在$\mathrm{1个}H/d$-$0.6V/C米$. 与对照相比，生物质、碳水化合物和脂质的生产率 (mgL ^-1 d ^{-1 ) 分别增加了 31%、55% 和 47%。}为了$\mathrm{1个}H/d$-$1.2V/C米$干预后，棕榈酸和棕榈油酸分别增加了 24.5% 和 45.9%。此外，脂肪酸显示出改进的生物柴油特性，包括长链饱和因子和冷滤点。编码糖酵解酶的基因：己糖激酶、磷酸丙糖异构酶、6-磷酸果糖激酶、丙酮酸脱氢酶上调或$\mathrm{1个}H/d$-$.\mathrm{2个}V/C米\text{.}$脂肪酸生物合成酶：脂肪酸合酶、酰基载体蛋白-S-丙二酰转移酶、3-氧代酰基-ACP-合酶 II、3-氧代酰基-ACP 还原酶、烯酰基-ACP 还原酶 I，上调下调$\mathrm{1个}H/d$-$0.6V/C米$，连同脂肪酸降解基因的下调。这项研究首次将 MEC 作为一种有效的刺激因素，促进Tribonema中的碳水化合物转化为脂肪酸，以生产环保且具有成本效益的能源。