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An adaptive three-dimensional hydrodynamic focusing microfluidic impedance flow cytometer
Analyst ( IF 3.6 ) Pub Date : 2023-05-30 , DOI: 10.1039/d3an00726j Yang Zhou 1, 2 , Jiao Wang 3 , Ting Liu 1 , Man Wu 1 , Yuwei Lan 1, 2 , Chunping Jia 1 , Jianlong Zhao 1, 2
Analyst ( IF 3.6 ) Pub Date : 2023-05-30 , DOI: 10.1039/d3an00726j Yang Zhou 1, 2 , Jiao Wang 3 , Ting Liu 1 , Man Wu 1 , Yuwei Lan 1, 2 , Chunping Jia 1 , Jianlong Zhao 1, 2
Affiliation
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Microfluidic impedance cytometry is emerging as a label-free, low-cost and portable solution for cell analysis. Impedance-based cell or particle characterization is provided by microfluidic and electronic devices. We report the design and characterization of a miniaturized flow cytometer based on a 3-dimensional (3D) hydrodynamic focusing mechanism. A sheath adaptively concentrated the sample laterally and vertically at the bottom of the microchannel, reducing the variance of particle translocation height and increasing the signal-to-noise ratio of the particle impedance pulse. Through simulation and confocal microscopy experiments, it has been verified that an increase in the ratio of sheath to sample decreased the cross-sectional area of the concentrated stream, which can be reduced to 26.50% of the pre-focusing value. The appropriate sheath flow settings increased the impedance pulse amplitude for different particles, and the coefficient of variation reduced by at least 35.85%, contributing to a more accurate representation of the particle impedance characteristic distribution. The system displayed the difference of HepG2 cell impedance before and after drug treatment, which is consistent with the results of flow cytometry, providing a convenient and inexpensive solution for monitoring cell status.
中文翻译：
自适应三维流体动力聚焦微流控阻抗流式细胞仪
微流控阻抗细胞术正在成为一种无标记、低成本且便携式的细胞分析解决方案。基于阻抗的细胞或颗粒表征由微流体和电子设备提供。我们报告了基于 3 维 (3D) 流体动力学聚焦机制的小型流式细胞仪的设计和表征。鞘层自适应地将样品横向和垂直集中在微通道底部，减少了颗粒移位高度的方差，提高了颗粒阻抗脉冲的信噪比。通过模拟和共焦显微镜实验，验证了鞘与样品的比例增加，会减小聚光流的截面积，可减小至预聚焦值的26.50%。适当的鞘流设置增加了不同颗粒的阻抗脉冲幅度，变异系数至少降低了35.85%，有助于更准确地表示颗粒阻抗特征分布。该系统显示了药物治疗前后HepG2细胞阻抗的差异，与流式细胞术的结果一致，为监测细胞状态提供了一种方便且廉价的解决方案。
更新日期：2023-05-30
中文翻译：
自适应三维流体动力聚焦微流控阻抗流式细胞仪
微流控阻抗细胞术正在成为一种无标记、低成本且便携式的细胞分析解决方案。基于阻抗的细胞或颗粒表征由微流体和电子设备提供。我们报告了基于 3 维 (3D) 流体动力学聚焦机制的小型流式细胞仪的设计和表征。鞘层自适应地将样品横向和垂直集中在微通道底部，减少了颗粒移位高度的方差，提高了颗粒阻抗脉冲的信噪比。通过模拟和共焦显微镜实验，验证了鞘与样品的比例增加，会减小聚光流的截面积，可减小至预聚焦值的26.50%。适当的鞘流设置增加了不同颗粒的阻抗脉冲幅度，变异系数至少降低了35.85%，有助于更准确地表示颗粒阻抗特征分布。该系统显示了药物治疗前后HepG2细胞阻抗的差异，与流式细胞术的结果一致，为监测细胞状态提供了一种方便且廉价的解决方案。
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