Biological Transformation of AgI on MOF-on-MOF-Derived Heterostructures: Toward Polarity-Switchable Photoelectrochemical Biosensors for Neuron-Specific Enolase,Analytical Chemistry

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Biological Transformation of AgI on MOF-on-MOF-Derived Heterostructures: Toward Polarity-Switchable Photoelectrochemical Biosensors for Neuron-Specific Enolase
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-05-30 , DOI: 10.1021/acs.analchem.3c01401
Feng-Zao Chen _{1,

2} , Xiao-Xue Fu _{1,

3} , Xiao-Jie Yu ₂ , Yu-Hang Qiu ₃ , Shi-Bin Ren ₂ , Yi-Chao Wang ₁ , De-Man Han ₂ , Wei-Wei Zhao ₃

Affiliation

The sensitive detection of neuron-specific enolase (NSE) as a biomarker for lung cancer at an early stage is critical but has long been a challenge. The emergence of polarity-switchable photoelectrochemical (PEC) bioanalysis has opened up new avenues for developing highly sensitive NSE sensors. In this study, we present such a biosensor depending on the bioinduced AgI transition on MOF-on-MOF-derived semiconductor heterojunctions. Specifically, treatment of ZnO@In₂O₃@AgI by bioproduced H₂S can in situ generate the ZnO@In₂O₃@In₂S₃@Ag₂S heterojunction, with the photocurrent switching from the cathodic to anodic one due to the changes in the carrier transfer pathway. Linking an NSE-targeted sandwich immunorecognition with labeled alkaline phosphatase (ALP) catalyzed generation of H₂S, such a phenomenon was correlated to NSE concentration with good performance in terms of selectivity and sensitivity and a low detection limit of 0.58 pg/mL. This study offered a new perspective on the use of MOF-on-MOF-derived heterostructures for advanced polarity-switchable PEC bioanalysis.

中文翻译：

AgI 在 MOF-on-MOF 衍生的异质结构上的生物转化：神经元特异性烯醇化酶的极性可切换光电化学生物传感器

神经元特异性烯醇化酶 (NSE) 作为早期肺癌生物标志物的灵敏检测至关重要，但长期以来一直是一个挑战。极性可切换光电化学 (PEC) 生物分析的出现为开发高灵敏度 NSE 传感器开辟了新途径。在这项研究中，我们根据 MOF-on-MOF 衍生的半导体异质结上的生物诱导 AgI 转变提出了这样一种生物传感器。具体而言，通过生物产生的 H ₂ S处理 ZnO@In ₂ O ₃ @AgI可以原位生成 ZnO@In ₂ O ₃ @In ₂ S ₃ @Ag ₂S 异质结，由于载流子传输路径的变化，光电流从阴极切换到阳极。将 NSE 靶向夹心免疫识别与标记碱性磷酸酶 (ALP) 催化生成 H ₂ S 联系起来，这种现象与 NSE 浓度相关，在选择性和灵敏度方面表现良好，检测限低至 0.58 pg/mL。这项研究为使用 MOF-on-MOF 衍生的异质结构进行先进的极性可切换 PEC 生物分析提供了新的视角。

更新日期：2023-05-30

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