A reliable tool for real-time monitoring and quantifying the fluctuation of adenosine triphosphate (ATP) in living organisms is highly desired for further understanding its biological function. However, there is no probe available to trace ATP in vivo for the challenges of developing reversible responsive ratiometric probes in the near-infrared region with spectrum crosstalk-free. Here, the strategies to overcome above problems by elongating the π-conjugation system of the rhodamine skeleton and rational connecting the internal reference dyes are proposed to obtain the two proof-of-concept probes and . displays the NIR ratio emission signal (I/I) as well as over 200 nm wavelength differences between the two peaks after rapidly responding to ATP, which will greatly improve the imaging resolution and reliability. The confocal imaging experiments enables us to directly monitor the dynamic changes of mitochondrial ATP. Importantly, fills the gap in recognizing ATP in tumor tissues in a reversible way, and could distinguish and quantify ATP content in tumor tissue and normal tissue. Thus, we believe that has the potential as a useful molecular imaging tool to study of ATP physiological and pathological functions at the clinical level.

中文翻译：

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构建无发射串扰的近红外比率荧光探针，用于线粒体和肿瘤中的可逆 ATP 追踪


为了进一步了解其生物学功能，迫切需要一种可靠的工具来实时监测和量化活体中三磷酸腺苷（ATP）的波动。然而，由于在近红外区域开发无光谱串扰的可逆响应比率探针，目前还没有可用于体内追踪 ATP 的探针。在这里，提出了通过延长罗丹明骨架的π-共轭体系和合理连接内参染料来克服上述问题的策略，以获得两个概念验证探针 和 。显示近红外比发射信号（I/I）以及快速响应 ATP 后两个峰之间超过 200 nm 的波长差异，这将大大提高成像分辨率和可靠性。共聚焦成像实验使我们能够直接监测线粒体ATP的动态变化。重要的是，填补了以可逆方式识别肿瘤组织中ATP的空白，并且可以区分和量化肿瘤组织和正常组织中的ATP含量。因此，我们相信它有潜力成为一种有用的分子成像工具，用于在临床水平上研究 ATP 生理和病理功能。