High-Sensitivity Detection toward SARS-CoV-2 S1 Glycoprotein by Parallel Reaction Monitoring Mass Spectrometry,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

High-Sensitivity Detection toward SARS-CoV-2 S1 Glycoprotein by Parallel Reaction Monitoring Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-05-29 , DOI: 10.1021/acs.analchem.2c05770
Wen Zhou _{1,

2} , Zhigang Sui ₁ , Jianhui Liu ₁ , Yingyun He _{1,

2} , Huiming Yuan ₁ , Yue Sun _{1,

2} , Zhen Liang ₁ , Kaiguang Yang ₁ , Lihua Zhang ₁ , Yukui Zhang ₁

Affiliation

The outbreak of coronavirus disease 2019 (COVID-19) has overwhelmed the global economy and human well-being. On account of the sharp increase in test demand, there is a need for an accurate and alternative diagnosis method for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, with the aim to specifically identify the trace SARS-CoV-2 S1 glycoprotein, we developed a high-sensitivity and high-selectivity diagnostic method based on the targeted parallel reaction monitoring (PRM) assay of eight selected peptides. This study emphasizes the outstanding detection sensitivity of 0.01 pg of the SARS-CoV-2 S1 glycoprotein even in the interference of other structural proteins, which to our knowledge is the current minimum limit of detection for the SARS-CoV-2 S1 glycoprotein. This technology could further identify 0.01 pg of the SARS-CoV-2 S1 glycoprotein in a spike pseudovirus, revealing its practical effectiveness. All our preliminary results throw light on the capability of the mass spectrometry-based targeted PRM assay to identify SARS-CoV-2 as a practicable orthogonal diagnostic tool. Furthermore, this technology could be extended to other pathogens (e.g., MERS-CoV S1 protein or SARS-CoV S1 protein) by quickly adjusting the targeted peptides of MS data acquisition. In summary, this strategy is universal and flexible and could be quickly adjusted to detect and discriminate different mutants and pathogens.

中文翻译：

通过平行反应监测质谱法对 SARS-CoV-2 S1 糖蛋白进行高灵敏度检测

2019 年冠状病毒病 (COVID-19) 的爆发给全球经济和人类福祉带来了沉重打击。由于检测需求急剧增加，需要一种针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的准确且替代的诊断方法。在本研究中，为了特异性识别痕量 SARS-CoV-2 S1 糖蛋白，我们开发了一种基于八种选定肽的靶向平行反应监测 (PRM) 检测的高灵敏度和高选择性诊断方法。这项研究强调了 SARS-CoV-2 S1 糖蛋白即使在其他结构蛋白的干扰下也具有 0.01 pg 的出色检测灵敏度，据我们所知，这是目前 SARS-CoV-2 S1 糖蛋白的最低检测限。该技术可以进一步识别刺突假病毒中0.01 pg的SARS-CoV-2 S1糖蛋白，揭示其实际有效性。我们所有的初步结果都揭示了基于质谱的靶向 PRM 检测将 SARS-CoV-2 识别为实用的正交诊断工具的能力。此外，通过快速调整MS数据采集的目标肽，该技术可以扩展到其他病原体（例如MERS-CoV S1蛋白或SARS-CoV S1蛋白）。综上所述，该策略具有通用性和灵活性，可以快速调整以检测和区分不同的突变体和病原体。

更新日期：2023-05-29

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11