Boron neutron capture therapy (BNCT) has emerged as a treatment modality with high precision and efficacy of intractable tumors. At the core of effective tumor BNCT are ¹⁰B carriers with facile preparation as well as advantageous pharmacokinetic and therapeutic profiles. Herein, the design and preparation of sub-10 nm ¹⁰B-enriched hexagonal boron nitride nanoparticles grafted with poly(glycerol) (h-¹⁰BN-PG), and their application to cancer treatment by BNCT are reported. By virtue of their small particle size and outstanding stealth property, h-¹⁰BN-PG nanoparticles accumulate efficiently in murine CT26 colon tumors with a high intratumor ¹⁰B concentration of 8.8%ID g⁻¹ or 102.1 µg g⁻¹ at 12 h post-injection. Moreover, h-¹⁰BN-PG nanoparticles penetrate into the inside of the tumor parenchyma and then are taken up by the tumor cells. BNCT comprising a single bolus injection of h-¹⁰BN-PG nanoparticles and subsequent one-time neutron irradiation results in significant shrinkage of subcutaneous CT26 tumors. h-¹⁰BN-PG-mediated BNCT not only causes direct DNA damage to the tumor cells, but also triggers pronounced inflammatory immune response in the tumor tissues, which contributes to long-lasting tumor suppression after the neutron irradiation. Thus, the h-¹⁰BN-PG nanoparticles are promising BNCT agents to eradicate tumor through highly efficient ¹⁰B accumulation.

中文翻译：

---

富含 10B 的六方氮化硼纳米颗粒接枝聚甘油的硼中子俘获疗法根除肿瘤

硼中子俘获疗法（BNCT）已成为治疗顽固性肿瘤的一种高精度、高效的治疗方式。有效的肿瘤 BNCT 的核心是^{10 种}B 载体，其制备简便且具有有利的药代动力学和治疗特性。^{本文报道了接枝聚甘油（h- 10} BN-PG）的亚10 nm  ¹⁰ B富集六方氮化硼纳米颗粒的设计和制备，以及它们在BNCT癌症治疗中的应用。凭借其小粒径和出色的隐形特性，h- ¹⁰ BN-PG纳米粒子在小鼠CT26结肠肿瘤中有效积累，12小时后肿瘤内¹⁰ B浓度高达8.8%ID g ⁻¹或102.1 µg g ⁻¹ -注射。此外，h- ¹⁰ BN-PG纳米颗粒渗透到肿瘤实质内部，然后被肿瘤细胞摄取。BNCT 包括单次推注 h- ¹⁰ BN-PG 纳米粒子和随后的一次性中子照射，导致皮下 CT26 肿瘤显着缩小。h- ¹⁰ BN-PG介导的BNCT不仅对肿瘤细胞造成直接DNA损伤，而且在肿瘤组织中引发明显的炎症免疫反应，这有助于中子照射后持久的肿瘤抑制。因此，h- ¹⁰ BN-PG纳米粒子是有前途的BNCT制剂，可通过高效的¹⁰ B积累来根除肿瘤。