Genome-wide phenotypic screens in the budding yeast Saccharomyces cerevisiae , enabled by its knockout collection, have produced the largest, richest, and most systematic phenotypic description of any organism. However, integrative analyses of this rich data source have been virtually impossible because of the lack of a central data repository and consistent metadata annotations. Here, we describe the aggregation, harmonization, and analysis of ~14,500 yeast knockout screens, which we call Yeast Phenome. Using this unique dataset, we characterized two unknown genes ( YHR045W and YGL117W ) and showed that tryptophan starvation is a by-product of many chemical treatments. Furthermore, we uncovered an exponential relationship between phenotypic similarity and intergenic distance, which suggests that gene positions in both yeast and human genomes are optimized for function.

中文翻译：

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酵母敲除现象的整体分析


芽殖酵母的全基因组表型筛选酿酒酵母凭借其淘汰收集，已经对任何生物体产生了最大、最丰富和最系统的表型描述。然而，由于缺乏中央数据存储库和一致的元数据注释，对这一丰富数据源的综合分析实际上是不可能的。在这里，我们描述了约 14,500 个酵母敲除筛选的聚合、协调和分析，我们将其称为酵母表型组。使用这个独特的数据集，我们表征了两个未知基因（ YHR045W和YGL117W ）并表明色氨酸饥饿是许多化学处理的副产品。此外，我们发现表型相似性和基因间距离之间存在指数关系，这表明酵母和人类基因组中的基因位置都针对功能进行了优化。