Thousands of long-distance mobile mRNAs were identified from different grafting systems, based on high-throughput sequencing technology. Moreover, the long-distance delivery of RNAs was proved to involve multiple mechanisms. Here, we analyzed the homology, motif, and tRNA-like structure (TLS) of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein (RBP) in nine grafting combinations including Arabidopsis thaliana, Vitis vinifera, Cucumis sativus, Citrullus lanatus, Nicotiana benthamiana, Malus domestica, Pyrus spp., Glycine max and Phaseolus vulgaris. Although several mRNAs were found to be shared in herbaceous, woody, and related species, the vast majority of long-distance mobile mRNAs were species-specific. Four non-specific movement-related motifs were identified, while the TLS was not necessary for mRNA long distance mobility. In addition, we found that RBPs were conserved among herbaceous and woody plants as well as related species. This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable mRNAs were selected by bioinformatics, in order to provide insights for future research on long-distance mobile mRNAs.

基于高通量测序技术，从不同的移植系统中鉴定出了数千个长距离移动的 mRNA。此外，RNA的长距离递送被证明涉及多种机制。在这里，我们分析了通过 RNA-seq 鉴定的长距离移动 mRNA 的同源性、基序和 tRNA 样结构 (TLS)，以及包括拟南芥、葡萄在内的 9 个嫁接组合中的 RNA 结合蛋白 ( RBP )。、黄瓜、西瓜、烟草、海棠、梨属、大豆和菜豆。尽管发现一些 mRNA 在草本、木本和相关物种中是共享的，但绝大多数长距离移动 mRNA 是物种特异性的。鉴定出四个非特异性运动相关基序，而 TLS 对于 mRNA 长距离移动来说并不是必需的。此外，我们发现 RBP 在草本和木本植物以及相关物种中是保守的。本文进一步深入分析了生物信息学选择物种特异性可转运mRNA的内源机制，以期为未来长距离移动mRNA的研究提供思路。