Peptide Oxidation Induced by Liquid–Solid Contact Electrification as Revealed in Liquid Microjunction-Surface Sampling Probe Mass Spectrometry,Analytical Chemistry

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Peptide Oxidation Induced by Liquid–Solid Contact Electrification as Revealed in Liquid Microjunction-Surface Sampling Probe Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-05-25 , DOI: 10.1021/acs.analchem.3c01411
T-Y Lui ₁ , Xiangfeng Chen _{1,

2} , Simin Zhang ₁ , Danna Hu ₁ , T-W Dominic Chan ₁

Affiliation

Reproducible peptide oxidation was observed using a homebuilt liquid microjunction-surface sampling probe (LMJ-SSP) platform for analyzing peptide standards. Although electrochemical oxidation and corona discharges have previously been associated with analyte oxidation in electrospray ionization (ESI) and ESI-related ambient ionization mass spectrometry (MS) methods, they were unlikely the causes for the peptide oxidation observed in the LMJ-SSP studies. A systematic investigation demonstrated that analyte oxidation was induced during the droplet drying on a solid surface through liquid–solid electrification processes. To minimize unwanted analyte oxidation, the water content in the sample solution should be decreased and the use of hydroxyl-functionalized substrates, such as glass slides, should be avoided. In addition, if water is an essential solvent component, adding an antioxidant, such as ascorbic acid, to the sample solution before droplet evaporation on the solid surface could lower the percentage of analyte oxidation. The present findings apply to all the MS methods that involve drying microliters of sample solution onto a suitable substrate in their sample preparation protocols.

中文翻译：

液-固接触带电诱导的肽氧化在液体微连接-表面取样探针质谱中的揭示

使用用于分析肽标准品的自制液体微连接表面采样探针 (LMJ-SSP) 平台观察到可重现的肽氧化。尽管电化学氧化和电晕放电以前与电喷雾电离 (ESI) 和 ESI 相关的环境电离质谱 (MS) 方法中的分析物氧化有关，但它们不太可能是 LMJ-SSP 研究中观察到的肽氧化的原因。一项系统的研究表明，通过液-固带电过程，在固体表面上的液滴干燥过程中会引起分析物氧化。为了尽量减少不需要的分析物氧化，应降低样品溶液中的水含量，并应避免使用羟基功能化的基板，例如载玻片。此外，如果水是必不可少的溶剂成分，则在固体表面上的液滴蒸发之前向样品溶液中添加抗氧化剂（例如抗坏血酸）可以降低分析物氧化的百分比。目前的发现适用于所有 MS 方法，这些方法涉及在其样品制备方案中将微升样品溶液干燥到合适的基质上。

更新日期：2023-05-25

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