Chronic hard-to-heal wounds draw great attention worldwide, as their treatments are limited by infections and hypoxia. Inspired by the natural oxygen production capacity of algae and the competitive advantage of beneficial bacteria over other microbes, we presented a living microecological hydrogel (LMH) with functionalized Chlorella and Bacillus subtilis encapsulation to realize continuous oxygen delivery and anti-infections for promoting chronic wound healing. As the hydrogel consisted of thermosensitive Pluronic F-127 and wet-adhesive polydopamine, the LMH could keep liquid at a low temperature while quickly solidifying and tightly adhering to the wound bed. It was demonstrated that by optimizing the proportion of the encapsulated microorganism, the Chlorella could continuously produce oxygen to relieve hypoxia and support the proliferation of B. subtilis , while B. subtilis could eliminate the colonized pathogenic bacteria. Thus, the LMH substantially promoted the healing of infected diabetic wounds. These features make the LMH valuable for practical clinical applications.

中文翻译：

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用于伤口愈合的活性微生态水凝胶

慢性难以愈合的伤口引起了全世界的极大关注，因为它们的治疗受到感染和缺氧的限制。受藻类天然产氧能力和有益细菌相对于其他微生物的竞争优势的启发，我们提出了一种功能化的活微生态水凝胶 (LMH)小球藻和枯草杆菌封装实现持续供氧和抗感染，促进慢性伤口愈合。由于水凝胶由热敏性 Pluronic F-127 和湿粘合剂聚多巴胺组成，因此 LMH 可以在低温下保持液态，同时快速凝固并紧密粘附在伤口床上。结果表明，通过优化封装微生物的比例，小球藻可以持续产生氧气以缓解缺氧并支持增殖枯草芽孢杆菌， 尽管枯草芽孢杆菌能消灭定植的致病菌。因此，LMH 大大促进了感染糖尿病伤口的愈合。这些特性使 LMH 对实际临床应用很有价值。