Phthalic acid esters (PAEs) are known as the most widely used plasticizer as well as one of the ubiquitously distributed emerging pollutants. Biodegradation and bioremediation via application of PAEs-degrading microbes is promising. In this study, a novel marine microbe, Gordonia hongkongensis RL-LY01, was isolated from mangrove sediment showing high di-(2-ethylhexyl) phthalate (DEHP) degradation capacity. Strain RL-LY01 could degrade a wide range of PAEs and the degradation kinetics of DEHP followed the first-order decay model. Meanwhile, good environmental adaptability, preference to alkaline conditions and good tolerance to salinity and metal ions was shown. Further, metabolic pathway of DEHP in strain RL-LY01 was proposed, with di-ethyl phthalate, phthalic acid, benzoic acid and catechol as intermediates. Additionally, one known mono-alkyl phthalate hydrolase gene (mehpH) was identified. Finally, the excellent performance during bioremediation of artificial DEHP-contaminated saline soil and sediment indicated strain RL-LY01 employs great application potential for the bioremediation of PAE-contaminated environments.

邻苯二甲酸酯（PAEs）被称为最广泛使用的增塑剂，也是无处不在的新兴污染物之一。通过应用 PAEs 降解微生物进行生物降解和生物修复是有前途的。在这项研究中，一种新型海洋微生物Gordonia hongkongensisRL-LY01 是从红树林沉积物中分离出来的，具有很高的邻苯二甲酸二（2-乙基己基）酯 (DEHP) 降解能力。菌株 RL-LY01 可以降解多种 PAE，DEHP 的降解动力学遵循一级衰减模型。同时表现出良好的环境适应性，喜碱性条件，对盐度和金属离子具有良好的耐受性。此外，还提出了菌株 RL-LY01 中 DEHP 的代谢途径，其中邻苯二甲酸二乙酯、邻苯二甲酸、苯甲酸和儿茶酚为中间体。此外，一种已知的邻苯二甲酸单烷基酯水解酶基因 ( mehpH) 被识别出来。最后，人工DEHP污染的盐渍土和沉积物生物修复过程中的优异性能表明菌株RL-LY01在PAE污染环境的生物修复中具有巨大的应用潜力。