In this study, Fe₂O₃ nanoparticles (Fe₂O₃ NPs) and CaO NPs were loaded on the zeolite sphere carrier to create nano Fe-Ca bimetallic oxide (Fe-Ca-NBMO) modified substrate, which was introduced into constructed wetland (CW) to remove Cu(II) and Ni(II) via constructing "substrate-microorganism" system. Adsorption experiments showed that the equilibrium adsorption capacities of Fe-Ca-NBMO modified substrate for Cu(II) and Ni(II) were respectively 706.48 and 410.59 mg/kg at an initial concentration of 20 mg/L, 2.45 and 2.39 times of gravel. The Cu(II) and Ni(II) removal efficiencies in CW with Fe-Ca-NBMO modified substrate respectively reached 99.7% and 99.9% at an influent concentration of 100 mg/L, significantly higher than those in gravel-based CW (47.0% and 34.3%). Fe-Ca-NBMO modified substrate could promote Cu(II) and Ni(II) removal by increasing electrostatic adsorption, chemical precipitation, as well as the abundances of resistant microorganisms (Geobacter, Desulfuromonas, Zoogloea, Dechloromonas, and Desulfobacter) and functional genes (copA, cusABC, ABC.CD.P, gshB, and exbB). This study provided an effective method to enhance Cu(II) and Ni(II) removal of electroplating wastewater by CW with Fe-Ca-NBMO modified substrate.

在这项研究中，Fe ₂ O ₃纳米颗粒（Fe ₂ O ₃NPs) 和 CaO NPs 负载在沸石球载体上以制备纳米 Fe-Ca 双金属氧化物 (Fe-Ca-NBMO) 改性基材，将其引入人工湿地 (CW) 以去除 Cu(II) 和 Ni(II)通过构建“底物-微生物”系统。吸附实验表明，Fe-Ca-NBMO改性基质对Cu(II)和Ni(II)的平衡吸附量分别为706.48和410.59 mg/kg，初始浓度为20 mg/L，是砾石的2.45和2.39倍. 在进水浓度为 100 mg/L 时，Fe-Ca-NBMO 改性基质对 CW 中 Cu(II) 和 Ni(II) 的去除效率分别达到 99.7% 和 99.9%，显着高于砾石基 CW（47.0 % 和 34.3%)。Fe-Ca-NBMO改性底物可通过增加静电吸附促进Cu(II)和Ni(II)的去除，Geobacter、Desulfurononas、Zoogloea、Dechloromonas和Desulfobacter）和功能基因（copA、cusABC、ABC.CD.P、gshB和exbB）。本研究提供了一种有效的方法来提高 Fe-Ca-NBMO 改性底物 CW 对电镀废水中 Cu(II) 和 Ni(II) 的去除率。