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Three-dimensional label-free visualization of the interactions of PM2.5 with macrophages and epithelial cells using optical diffraction tomography
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-05-23 , DOI: 10.1016/j.jhazmat.2023.131678
Wang Sik Lee 1 , Inha Kang 2 , Sung-Jin Yoon 1 , Hyunjung Kim 1 , Yugyeong Sim 3 , Youngjun Park 1 , Jinah Park 2 , Jinyoung Jeong 3
Affiliation  

Particulate matter ≤ 2.5 µm (PM2.5) poses health risks related to various diseases and infections. However, the interactions between PM2.5 and cells such as uptake and cell responses have not been fully investigated despite advances in bioimaging techniques, because the heterogeneous morphology and composition of PM2.5 make it challenging to employ labeling techniques, such as fluorescence. In this work, we visualized the interaction between PM2.5 and cells using optical diffraction tomography (ODT), which provides quantitative phase images by refractive index distribution. Through ODT analysis, the interactions of PM2.5 with macrophages and epithelial cells, such as intracellular dynamics, uptake, and cellular behavior, were successfully visualized without labeling techniques. ODT analysis clearly shows the behavior of phagocytic macrophages and nonphagocytic epithelial cells for PM2.5. Moreover, ODT analysis could quantitatively compare the accumulation of PM2.5 inside the cells. PM2.5 uptake by macrophages increased substantially over time, but uptake by epithelial cells increased only marginally. Our findings indicate that ODT analysis is a promising alternative approach to visually and quantitatively understanding the interaction of PM2.5 with cells. Therefore, we expect ODT analysis to be employed to investigate the interactions of materials and cells that are difficult to label.



中文翻译:

使用光学衍射层析成像技术对 PM2.5 与巨噬细胞和上皮细胞的相互作用进行三维无标记可视化

≤ 2.5 µm (PM2.5) 的颗粒物会带来与各种疾病和感染相关的健康风险。然而,尽管生物成像技术取得了进步,但 PM2.5 与细胞之间的相互作用(例如摄取和细胞反应)尚未得到充分研究,因为 PM2.5 的异质形态和成分使得使用荧光等标记技术具有挑战性。在这项工作中,我们使用光学衍射层析成像 (ODT) 可视化 PM2.5 与细胞之间的相互作用,它通过折射率分布提供定量相位图像。通过 ODT 分析,PM2.5 与巨噬细胞和上皮细胞的相互作用,例如细胞内动力学、摄取和细胞行为,无需标记技术即可成功可视化。ODT 分析清楚地显示了吞噬性巨噬细胞和非吞噬性上皮细胞对 PM2.5 的行为。此外,ODT分析可以定量比较细胞内PM2.5的积累。随着时间的推移,巨噬细胞对 PM2.5 的吸收显着增加,但上皮细胞对 PM2.5 的吸收仅略有增加。我们的研究结果表明,ODT 分析是一种有前途的替代方法,可以从视觉和定量上了解 PM2.5 与细胞的相互作用。因此,我们希望使用 ODT 分析来研究难以标记的材料和细胞的相互作用。但上皮细胞的摄取仅略有增加。我们的研究结果表明,ODT 分析是一种有前途的替代方法,可以从视觉和定量上了解 PM2.5 与细胞的相互作用。因此,我们希望使用 ODT 分析来研究难以标记的材料和细胞的相互作用。但上皮细胞的摄取仅略有增加。我们的研究结果表明,ODT 分析是一种有前途的替代方法,可以从视觉和定量上了解 PM2.5 与细胞的相互作用。因此,我们希望使用 ODT 分析来研究难以标记的材料和细胞的相互作用。

更新日期:2023-05-26
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