Jujube witches’ broom (JWB) caused by phytoplasma has a severely negative effect on multiple metabolisms in jujube. The GST gene family in plants participates in the regulation of a variety of biotic and abiotic stresses. This study aims to identify and reveal the changes in the jujube GST gene family in response to phytoplasma infection. Here, 70 ZjGSTs were identified in the jujube genome and divided into 8 classes. Among them, the Tau-class, including 44 genes, was the largest. Phylogenetic analysis indicated that Tau-class genes were highly conserved among species, such as Arabidopsis, cotton, chickpea, and rice. Through chromosome location analysis, 37.1% of genes were clustered, and 8 of 9 gene clusters were composed of Tau class members. Through RT-PCR, qRT-PCR and enzyme activity detection, the results showed that the expression of half (20/40) of the tested ZjGSTs was inhibited by phytoplasma infection in field and tissue culture conditions, and GST activity was also significantly reduced. In the resistant and susceptible varieties under phytoplasma infection, ZjGSTU49-ZjGSTU54 in the cluster Ⅳ showed opposite expression patterns, which may be due to functional divergence during evolution. Some upregulated genes (ZjGSTU45, ZjGSTU49, ZjGSTU59, and ZjGSTU70) might be involved in the process of jujube against JWB. The yeast two-hybrid results showed that all 6 Tau-class proteins tested could form homodimers or heterodimers. Overall, the comprehensive analysis of the jujube GST gene family revealed that ZjGSTs responded actively to phytoplasma infection. Furthermore, some screened genes (ZjGSTU24, ZjGSTU49-52, ZjGSTU70, and ZjDHAR10) will contribute to further functional studies of jujube-phytoplasma interactions.

由植原体引起的枣巫帚病（JWB）对枣体内多种代谢产生严重的负面影响。植物中的GST基因家族参与多种生物和非生物胁迫的调节。本研究旨在鉴定并揭示枣 GST 基因家族响应植原体感染的变化。在此，在枣基因组中鉴定出 70 个ZjGST，并将其分为 8 类。其中，Tau类基因最多，包含44个基因。系统发育分析表明，Tau 类基因在拟南芥、棉花、鹰嘴豆和水稻等物种之间高度保守。通过染色体定位分析，37.1%的基因被聚类，9个基因簇中有8个由Tau类成员组成。通过RT-PCR、qRT-PCR和酶活性检测，结果显示，在田间和组织培养条件下，受试ZjGSTs中有一半（20/40）的表达受到植原体感染的抑制，GST活性也显着降低。在植原体感染下的抗性和感病品种中，簇IV中的ZjGSTU49 - ZjGSTU54表现出相反的表达模式，这可能是由于进化过程中的功能分歧所致。一些上调基因( ZjGSTU45、ZjGSTU49、ZjGSTU59和ZjGSTU70 )可能参与了枣抗JWB的过程。酵母双杂交结果显示，测试的6种Tau类蛋白均能形成同二聚体或异二聚体。总体而言，对枣GST基因家族的综合分析表明，ZjGSTs对植原体感染反应积极。此外，一些筛选的基因（ZjGSTU24 、 ZjGSTU49-52 、 ZjGSTU70和ZjDHAR10 ）将有助于枣与植原体相互作用的进一步功能研究。