Background

The flavonoids and polysaccharides in Portulaca oleracea L. (PO) have significant antibacterial and antioxidant effects, which can inhibit common bacteria and remove free radicals in the body. However, there was little research on the use of PO to alleviate hyperpigmentation and photoaging damage.

Purpose

This study was to investigate the anti-photoaging and whitening activity mechanism of polysaccharide of PO (POP) in vitro and in vivo.

Method

In this study, 16 fractions obtained by four enzyme-assisted extraction from PO and their scavenging capabilities against 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals were evaluated. Among these fractions, a polysaccharide fraction (VPOP3) showed the strongest biological activity. VPOP3 was characterized by Fourier-transform infrared spectroscopy, molecular weight (MW), and monosaccharide composition analysis, and the protective effect of VPOP3 on photoaging and hyperpigmentation was researched.

Results

VPOP3 is a low-MW acidic heteropolysaccharide with MW mainly distributed around 0.71KDa, arabinose as its main monosaccharide component. VPOP3 reliably reduced the reactive oxygen species levels in cells and zebrafish and the level of lipid peroxidation in zebrafish. In addition, VPOP3 inhibited UVB-induced apoptotic body formation and apoptosis by downregulating caspase-3 and Bax and upregulating Bcl-2 in mitochondrion-mediated signaling pathways. On the other hand, VPOP3 at high concentrations significantly downregulated the expression of microphthalmia-associated transcription factor, tyrosinase (TYR), and TYR-related protein-1 and TYR-related protein-2 in the melanogenic signaling pathway to achieve a whitening effect.

Conclusion

The above results showed that VPOP3 has superior activities of anti-photoaging and anti-melanogenesis and can be utilized as a safe resource in the manufacture of cosmetics.

中文翻译：

---

马齿苋不同酶辅助多糖提取物的抗黑色素生成和抗光老化活性的表征和筛选。

背景

马齿苋中的黄酮类和多糖。(PO)具有显着的抗菌和抗氧化作用，可抑制体内常见细菌，清除体内自由基。然而，关于使用 PO 减轻色素沉着过度和光老化损伤的研究很少。

目的

本研究旨在探讨 PO 多糖 (POP)在体外和体内的抗光老化和美白活性机制。

方法

在这项研究中，评估了通过四种酶辅助提取 PO 获得的 16 个馏分及其对 2,2-二苯基-1-苦基肼基和羟基自由基的清除能力。在这些级分中，多糖级分 (VPOP3) 显示出最强的生物活性。通过傅里叶变换红外光谱、分子量(MW)和单糖组成分析对VPOP3进行了表征，并研究了VPOP3对光老化和色素沉着过度的保护作用。

结果

VPOP3是一种分子量主要分布在0.71KDa左右的低分子量酸性杂多糖，其单糖主要成分为阿拉伯糖。VPOP3 可靠地降低了细胞和斑马鱼中的活性氧水平以及斑马鱼的脂质过氧化水平。此外，VPOP3 通过下调线粒体介导的信号通路中的 caspase-3 和 Bax 以及上调 Bcl-2 来抑制 UVB 诱导的凋亡小体形成和细胞凋亡。另一方面，高浓度VPOP3显着下调黑色素生成信号通路中小眼相关转录因子酪氨酸酶（TYR）、TYR相关蛋白1和TYR相关蛋白2的表达，从而达到美白效果。

结论

上述结果表明VPOP3具有优异的抗光老化和抗黑色素生成活性，可作为化妆品生产中的安全资源。