A new method of seed oil extraction from X. Sorbifolia was developed using a two-stage process with supercritical CO₂ (SC-CO₂) and CO₂-expanded ethanol (CXE). The BIC model was employed to optimize the extraction process. During the SC-CO₂ extraction stage, the CLSM imaging showed rapid release of EAO from the broken cells at the edge of the particles with an oil yield of 74.11% after 4 h. The follow-up CXE extraction at 10 MPa, 333 K, and 1.5 kg/h, with a CO₂ molar fraction of 0.5 resulted in 68% broken cells by SEM imaging, and obtained 18.42% of remaining HAO, with a total oil yield of 92.53%. The whole process was completed in 60% less time compared to the Soxhlet method. Lipid composition analysis found a small amount of fatty acid ethyl ester in CXE oil and a higher content of functional fatty acid nervonic acid at 4.12%.

使用超临界 CO ₂ (SC-CO ₂ ) 和 CO ₂膨胀乙醇 (CXE)的两阶段工艺开发了一种从文冠果中提取种子油的新方法。BIC模型用于优化提取过程。在SC-CO ₂提取阶段，CLSM成像显示EAO从颗粒边缘的破碎细胞中快速释放，4小时后出油率为74.11%。后续 CXE 萃取在 10 MPa、333 K 和 1.5 kg/h 下，使用 CO ₂0.5的摩尔分数导致68%的SEM成像破碎细胞，并获得18.42%的剩余HAO，总油率为92.53%。与索氏提取法相比，整个过程的完成时间缩短了 60%。脂质成分分析发现CXE油中含有少量脂肪酸乙酯，功能性脂肪酸神经酸含量较高，为4.12%。