This study was undertaken to analyse the potential bioactivities including antibacterial, antioxidant and antidiabetic derived from the methanolic extract and the column chromatography ethyl acetate fraction (AcOEt Fr) of Horsfieldia spicata leaves. Methanolic extract and 4 other fractions was calculated for total phenol and flavonoid contents along with tested for antibacterial, antioxidant and antidiabetic properties. Interestingly, the AcOEt Fr had the highest value for total flavonoid content and the best antioxidant, and antidiabetic activities. Therefore, the AcOEt Fr was further separated using column chromatography technique for obtaining 9 selected fractions namely fraction 1 (F1) - fraction 9 (F9) which were further tested. The results showed that the AcOEt column chromatography fractions namely F2, F3, F4 and F6 had the best clear inhibition antibacterial value against all bacterial tested. In addition, these fractions also exhibited better Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) values than others. Antioxidant, 2,2-diphenylpicrylhydrazyl (DPPH) assayed indicated that AcOEt Fr had the strongest IC₅₀ value of 47.30 μg/mL. Further, F4 column chromatography fraction showed the best inhibition against α-Glucosidase enzyme related to antidiabetic activity with an IC₅₀ value of 6.11 μg/mL. Liquid chromatography tandem-mass spectrometry (LC/MS/MS) analysis identified that F4 derived from AcOEt fraction had several compounds belonging to the flavonoid and phenolics such as 3′,5-dihydroxy-7,4′-dimethoxyflavone, 5,7-dihydroxy-3-(4′-hydroxybenzyl)chromone, and Kadsurenin I.

中文翻译：

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霍斯菲尔德 (Roxb.) 叶提取物及其活性组分的抗菌、抗氧化和抗糖尿病潜力研究 J. Sinclair

本研究旨在分析霍斯菲尔德的甲醇提取物和柱层析乙酸乙酯组分 (AcOEt Fr) 的潜在生物活性，包括抗菌、抗氧化和抗糖尿病。树叶。计算了甲醇提取物和其他 4 种馏分的总酚和类黄酮含量，并测试了抗菌、抗氧化和抗糖尿病特性。有趣的是，AcOEt Fr 的总类黄酮含量最高，并且具有最佳的抗氧化和抗糖尿病活性。因此，使用柱层析技术进一步分离AcOEt Fr，以获得9个选定的级分，即级分1(F1)-级分9(F9)，并对其进行进一步测试。结果表明，AcOEt柱层析组分F2、F3、F4和F6对所有测试细菌均具有最佳的明确抑制抗菌值。此外，这些级分还表现出比其他级分更好的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 值。抗氧化剂, 2,₅₀值为 47.30 μg/mL。此外，F4柱层析组分显示出对与抗糖尿病活性相关的α-葡萄糖苷酶的最佳抑制作用，IC ₅₀值为6.11 μg/mL。液相色谱串联质谱（LC/MS/MS）分析表明，AcOEt 馏分中的 F4 含有多种属于黄酮类和酚类的化合物，如 3',5-二羟基-7,4'-二甲氧基黄酮、5,7-二羟基-3-(4'-羟基苄基)色酮和Kadsurenin I。