Now, using cryo-electron microscopy, Hu et al. have determined the structure of FKS1 from Saccharomyces cerevisiae. FKS1 consists of a wedge-shaped transmembrane (TM) domain of 17 helices, cytoplasmic GT and accessory (AC) domains, and several unstructured extracellular loops. The TM domain contains multiple bound lipids that probably contribute to stabilization, as well as a pair of pockets, one each on the intracellular and extracellular sides, that form a likely path for glucan translocation. The active site of FKS1 is located within a solvent-exposed chamber formed by the GT domain and the TM domain at the membrane–cytoplasm interface.

现在，胡等人使用冷冻电子显微镜。已经确定了来自酿酒酵母的 FKS1 的结构。FKS1 由 17 个螺旋的楔形跨膜 (TM) 结构域、细胞质 GT 和附属 (AC) 结构域以及几个非结构化细胞外环组成。TM 域包含多种可能有助于稳定的结合脂质，以及一对口袋，每个口袋位于细胞内和细胞外侧，形成葡聚糖易位的可能路径。FKS1 的活性位点位于由膜-细胞质界面处的 GT 结构域和 TM 结构域形成的溶剂暴露室内。