Stingless bees are essential pollinators in tropical ecosystems. Yet compared to their temperate zone counterparts, we know very little about how they respond to agriculture-associated land use, or how this effect is modulated by seasonal variation. Coffee, a dominant crop across the tropics, is grown under diverse management practices, making coffee agroecosystems an important context in which to investigate effects of agricultural management on stingless bees. We evaluated the performance and pollen diet of replicated experimental colonies of the stingless bee Scaptotrigona mexicana in three habitats in a coffee-dominated landscape in southern Chiapas, Mexico: a conventional sun coffee farm, a shaded organic coffee farm, and a forest fragment. We asked how habitat and seasonality influenced colony weight, forager activity, and pollen diet over one year. We placed eight experimental colonies in three habitats (three each in coffee farms; two in forest) and monitored them monthly for colony weight and forager activity. We collected corbicular pollen loads from returning foragers and identified pollen morphospecies. We compared performance and diet metrics across habitats and between wet and dry seasons. Colony performance was dramatically worse in the sun coffee farm; all sun coffee colonies died during the study. Colony performance did not differ between shade coffee and forest. Pollen diet richness was lower in the sun coffee colonies, but all habitats shared the same core pollen morphospecies. Across habitats, colony weight and activity and pollen diet richness decreased during the wet season. Our study suggests that conventional sun coffee cannot support stingless bee colonies, likely because of combined effects of agrochemical exposure and floral resource scarcity. By contrast, shade coffee is equivalent to forests in supporting stingless bee colonies.

无刺蜜蜂是热带生态系统中必不可少的传粉者。然而，与温带地区的同类相比，我们对它们如何响应与农业相关的土地利用，或者这种影响如何受季节变化调节知之甚少。咖啡是热带地区的主要作物，其种植方式多种多样，这使得咖啡农业生态系统成为研究农业管理对无刺蜜蜂影响的重要背景。我们评估了无刺蜜蜂Scaptotrigona mexicana的复制实验菌落的性能和花粉饮食在墨西哥恰帕斯州南部以咖啡为主的景观中的三个栖息地：一个传统的阳光咖啡农场、一个阴凉的有机咖啡农场和一个森林碎片。我们询问了栖息地和季节性在一年内如何影响蜂群重量、觅食活动和花粉饮食。我们在三个栖息地放置了八个实验性群体（咖啡农场各三个；森林中两个）并每月监测它们的群体重量和觅食者活动。我们从返回的觅食者那里收集了 corbicular 花粉量并确定了花粉形态. 我们比较了不同栖息地以及雨季和旱季之间的性能和饮食指标。太阳咖啡农场的菌落表现明显更差；所有 sun 咖啡菌落在研究期间死亡。树荫下的咖啡和森林之间的菌落表现没有差异。阳光下咖啡菌落的花粉饮食丰富度较低，但所有栖息地都具有相同的核心花粉形态种类。在整个栖息地，蜂群重量和活动以及花粉饮食丰富度在雨季都有所下降。我们的研究表明，传统的阳光咖啡不能支持无刺蜂群，这可能是因为接触农用化学品和花卉资源稀缺的综合影响。相比之下，遮荫咖啡在支持无刺蜂群方面相当于森林。