Sequential Hydrothermal Extraction (SeqHTE) provides a versatile platform to produce phytochemicals from potato peels. However, there is a lack of understanding of the SeqHTE reaction mechanisms and pathways. In this study, the chemistry of SeqHTE for the recovery of valuable bioactives from peels was investigated. The slightly more polar conditions of the first stage of SeqHTE favored the retrieval of free form polyphenols and extraction of polysaccharides and glycoalkaloids. In contrast, the second stage displayed preferential recovery of bound-type polyphenols and extraction of solanidine. The acid-catalyzed acyl bimolecular and the unimolecular nucleophilic substitution reaction mechanisms were identified. Additionally, the kinetics indicated that the ideal conditions to enhance bioactive recovery while reducing degradation were a temperature range from 140 ℃ to 160 ℃ and 170–190 ℃ for the first and second stage, respectively. The results demonstrated that SeqHTE offers a suitable environment to break down the peels for the recovery of structurally diverse bioactives.

顺序水热提取 (SeqHTE) 提供了一个多功能平台，可以从马铃薯皮中生产植物化学物质。然而，人们对 SeqHTE 反应机制和途径缺乏了解。在这项研究中，研究了用于从果皮中回收有价值的生物活性物质的 SeqHTE 的化学性质。SeqHTE 第一阶段稍微更极性的条件有利于游离形式多酚的回收以及多糖和配糖生物碱的提取。相反，第二阶段显示出结合型多酚的优先回收和茄碱的提取。酸催化的酰基双分子和单分子确定了亲核取代反应机制。此外，动力学表明，在减少降解的同时提高生物活性恢复的理想条件分别是第一阶段和第二阶段的温度范围为 140 ℃ 至 160 ℃ 和 170-190 ℃。结果表明，SeqHTE 提供了一个合适的环境来分解果皮，以回收结构多样的生物活性物质。