Potential nutritional strategies to prevent and reverse sarcopenia in aging process: role of fish oil-derived ω-3 polyunsaturated fatty acids, wheat oligopeptide and their combined intervention,Journal of Advanced Research

当前位置： X-MOL 学术 › J. Adv. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Potential nutritional strategies to prevent and reverse sarcopenia in aging process: role of fish oil-derived ω-3 polyunsaturated fatty acids, wheat oligopeptide and their combined intervention
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2023-04-13 , DOI: 10.1016/j.jare.2023.04.005
Da Pan ₁ , Ligang Yang ₁ , Xian Yang ₁ , Dengfeng Xu ₁ , Shaokang Wang ₂ , Han Gao ₃ , Hechun Liu ₄ , Hui Xia ₁ , Chao Yang ₅ , Yifei Lu ₁ , Jihan Sun ₁ , Yuanyuan Wang ₁ , Guiju Sun ₁

Affiliation

Introduction

Nutritional support is potentially considered an essential step to prevent muscle loss and enhance physical function in older adults.

Objectives

This study aimed to assess the role of potential nutritional strategies, i.e., fish oil-derived ω-3 polyunsaturated fatty acids (PUFAs), wheat oligopeptide and their combined intervention, in preventing and reversing sarcopenia in aging process.

Methods

One hundred 25-month-old Sprague-Dawley rats were randomly divided into 10 groups, and 10 newly purchased 6-month-old rats were included in young control group (n=10). Fish oil (200, 400 or 800 mg/kg body weight), wheat oligopeptide (100, 200 or 400 mg/kg body weight), fish oil + wheat oligopeptide (800 + 100, 400 + 200 or 200 + 400 mg/kg body weight) or the equal volume of solvent were administered daily by gavage for 10 weeks. The effects of these interventions on natural aging rats were evaluated.

Results

All intervention groups had a significant increase in muscle mass and grip strength and reduction in perirenal fat weight when compared to the aged control group (P<0.05). The results of biochemical parameters, magnetic resonance imaging, proteomics and western blot suggested that the combination of wheat oligopeptide and fish oil-derived ω-3 PUFA, especially group WFM 2 (400 + 200mg/kg body weight fish oil + wheat oligopeptide), was found to be more effective against aging-associated muscle loss than single intervention. Additionally, the interventions ameliorated fatty infiltration, muscle atrophy, and congestion in the intercellular matrix, and inflammatory cell infiltration in muscle tissue. The interventions also improved oxidative stress, anabolism, hormone levels, and inflammatory levels of skeletal muscle.

Conclusions

The combination of fish oil-derived ω-3 PUFA and wheat oligopeptide was found to be a promising nutritional support to prevent and reverse sarcopenia. The potential mechanism involved the promotion of protein synthesis and muscle regeneration, as well as the enhancement of muscle strength.

中文翻译：

预防和逆转衰老过程中肌肉减少症的潜在营养策略：鱼油衍生的ω-3多不饱和脂肪酸、小麦寡肽及其联合干预的作用

介绍

营养支持可能被认为是防止老年人肌肉损失和增强身体功能的重要步骤。

目标

本研究旨在评估潜在的营养策略，即鱼油衍生的ω-3多不饱和脂肪酸（PUFA）、小麦寡肽及其联合干预在预防和逆转衰老过程中肌肉减少症中的作用。

方法

100只25月龄Sprague-Dawley大鼠随机分为10组，其中10只新购6月龄大鼠为幼龄对照组（n=10）。鱼油（200、400或800毫克/公斤体重）、小麦寡肽（100、200或400毫克/公斤体重）、鱼油+小麦寡肽（800+100、400+200或200+400毫克/公斤）每天通过管饲法施用等体积的溶剂，持续 10 周。评估了这些干预措施对自然衰老大鼠的影响。

结果

与老年对照组相比，所有干预组的肌肉质量和握力均显着增加，肾周脂肪重量显着减少（ P <0.05）。生化参数、磁共振成像、蛋白质组学和蛋白质印迹的结果表明，小麦寡肽和鱼油衍生的ω-3 PUFA的组合，特别是WFM 2组（400+200mg/kg体重鱼油+小麦寡肽），研究发现，与单一干预相比，对抗衰老相关的肌肉损失更有效。此外，干预措施还改善了脂肪浸润、肌肉萎缩、细胞间基质充血以及肌肉组织中的炎症细胞浸润。这些干预措施还改善了骨骼肌的氧化应激、合成代谢、激素水平和炎症水平。