Apple latent spherical virus (ALSV; genus: Cheravirus, family: Secoviridae) is capable of infecting Angelica sinensis under natural conditions and causing symptoms of greenish discoloration and mottling, affecting plant growth and causing a reduction in root quality and yield. Virus detection of ALSV is an urgent need for the continued development of angelica industry. Consequently, it is necessary to develop simple, sensitive, and reliable ALSV detection methods. We describe the preparation of polyclonal antibodies with high titer and high specificity using recombinant ALSV coat protein (CP) as the antigen. Using these antibodies, we established and optimized a rapid detection system using colloidal gold immunochromatography assay (GICA) to detect ALSV. A real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) system with a minimum detection line of 2.72 × 10³copys/μl was also developed to detect ALSV. This assay was further enhanced using a combination GICA-RT-LAMP assay. This GICA assay successfully detected ALSV in infected plants without cross reactivity recorded from three other plant viruses. The sensitivity of GICA was the same as that of GICA-RT-PCR, whereas the sensitivity of GICA-RT-LAMP was at least 10-fold higher than that of GICA and GICA-RT-PCR. These methods can detect and identify ALSV under field conditions and accurately measure virus concentration in the laboratory.

苹果潜伏球形病毒（ALSV；属：Cheravirus，科：Secoviridae）能够感染当归在自然条件下，会引起绿色变色和斑点症状，影响植物生长，导致根系质量和产量下降。ALSV病毒检测是当归产业持续发展的迫切需要。因此，有必要开发简单、灵敏和可靠的 ALSV 检测方法。我们描述了使用重组 ALSV 外壳蛋白 (CP) 作为抗原制备具有高滴度和高特异性的多克隆抗体。使用这些抗体，我们建立并优化了使用胶体金免疫层析法 (GICA) 检测 ALSV 的快速检测系统。^{最小检测线为2.72×10 3}的实时逆转录环介导等温扩增（RT-LAMP）系统拷贝数/μl 也被开发用于检测 ALSV。使用组合 GICA-RT-LAMP 测定进一步增强了该测定。这种 GICA 检测成功地检测了受感染植物中的 ALSV，而没有从其他三种植物病毒中记录到交叉反应。GICA 的灵敏度与 GICA-RT-PCR 相同，而 GICA-RT-LAMP 的灵敏度至少比 GICA 和 GICA-RT-PCR 高 10 倍。这些方法可以在野外条件下检测和识别 ALSV，并在实验室中准确测量病毒浓度。