In this research article, we investigated the effect of Euphorbia bivonae extract compounds on the lethality of brine shrimp Artemia salina and on embryonic cell lines (HEK293) proliferation. Our GC/MS analysis revealed that the E. bivonae ethanolic extract contained essentially sitosterol, euphol, and lupeol. The 24-h LC50 was determined using the probit analysis method (LC50=357.11 mg l⁻¹). Depending on this cytotoxicity test result, E. bivona extract induced a significant increase in Superoxide Dismutase (SOD), Catalase (CAT), Glutathione-Peroxidase (GPx) activities, and lipid peroxidation (LPO) in A. salina larvae. In addition, the cytotoxicity effect of this extract had proved against the HEK293 cell lines in vitro. We suggest that the three compounds of E. bivonae extract (sitosterol, euphol, and lupeol) are the most responsible for this cytotoxicity. The possible application of this extract as an alternative natural antiproliferative is considered.

中文翻译：

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大戟提取物的植物化学成分及其对卤虫卤虫和胚胎肾 (HEK293) 细胞致死率的细胞毒性作用

在这篇研究文章中，我们研究了大戟提取物化合物对卤虫卤虫致死率和胚胎细胞系 (HEK293) 增殖的影响。我们的 GC/MS 分析表明，E. bivonae乙醇提取物主要含有谷甾醇、大戟酚和羽扇豆醇。使用概率单位分析法测定 24 小时 LC50 (LC50=357.11 mg l ^-1 )。根据该细胞毒性测试结果，E. bivona提取物可显着增加 A. salina 中的超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GPx) 活性和脂质过氧化 (LPO )幼虫。此外，该提取物在体外对 HEK293 细胞系具有细胞毒性作用。我们认为E. bivonae提取物的三种化合物（谷甾醇、大戟酚和羽扇豆醇）是造成这种细胞毒性的主要原因。考虑将该提取物作为替代天然抗增殖剂的可能应用。