Biosensing based on CRISPR-Cas systems is a young but rapidly evolving technology. The unprecedented properties of the CRISPR-Cas system provide an innovative tool for developing new-generation biosensing strategies. To date, a series of nucleic acid and non-nucleic acid detection methods have been developed based on the CRISPR platform. In this review, we first introduce the core biochemical properties underpinning the development of CRISPR bioassays, such as diverse reaction temperatures, programmability in design, high reaction efficiency, and recognition specificity, and highlight recent efforts to improve these parameters. We then introduce the technical developments, including how to improve sensitivity and quantification capabilities, develop multiplex assays, achieve convenient one-pot assays, create advanced sensors, and extend the applications of detection. Finally, we analyze obstacles to the commercial application of CRISPR detection technology and explore development opportunities and directions.

中文翻译：

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基于CRISPR的生物传感策略：技术发展与应用前景


基于 CRISPR-Cas 系统的生物传感是一项年轻但发展迅速的技术。 CRISPR-Cas系统前所未有的特性为开发新一代生物传感策略提供了创新工具。目前，基于CRISPR平台已开发出一系列核酸和非核酸检测方法。在这篇综述中，我们首先介绍了支撑 CRISPR 生物测定发展的核心生化特性，例如不同的反应温度、设计的可编程性、高反应效率和识别特异性，并重点介绍了最近为改进这些参数所做的努力。然后我们介绍了技术进展，包括如何提高灵敏度和定量能力、开发多重检测、实现便捷的一锅检测、创建先进的传感器以及扩展检测应用。最后，我们分析了CRISPR检测技术商业化应用的障碍，并探讨了发展机会和方向。