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Poaceae-specific β-1,3;1,4-d-glucans link jasmonate signalling to OsLecRK1-mediated defence response during rice-brown planthopper interactions
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2023-03-23 , DOI: 10.1111/pbi.14038
Yang-Shuo Dai 1 , Di Liu 1 , Wuxiu Guo 1 , Zhi-Xuan Liu 2 , Xue Zhang 1 , Li-Li Shi 1 , De-Mian Zhou 1 , Ling-Na Wang 1 , Kui Kang 1 , Feng-Zhu Wang 1 , Shan-Shan Zhao 1 , Yi-Fang Tan 1 , Tian Hu 1 , Wu Chen 2 , Peng Li 2 , Qing-Ming Zhou 2 , Long-Yu Yuan 3 , Zhenfei Zhang 3 , Yue-Qin Chen 1 , Wen-Qing Zhang 1 , Juan Li 2 , Lu-Jun Yu 1 , Shi Xiao 1
Affiliation  

Brown planthopper (BPH, Nilaparvata lugens), a highly destructive insect pest, poses a serious threat to rice (Oryza sativa) production worldwide. Jasmonates are key phytohormones that regulate plant defences against BPH; however, the molecular link between jasmonates and BPH responses in rice remains largely unknown. Here, we discovered a Poaceae-specific metabolite, mixed-linkage β-1,3;1,4-d-glucan (MLG), which contributes to jasmonate-mediated BPH resistance. MLG levels in rice significantly increased upon BPH attack. Overexpressing OsCslF6, which encodes a glucan synthase that catalyses MLG biosynthesis, significantly enhanced BPH resistance and cell wall thickness in vascular bundles, whereas knockout of OsCslF6 reduced BPH resistance and vascular wall thickness. OsMYC2, a master transcription factor of jasmonate signalling, directly controlled the upregulation of OsCslF6 in response to BPH feeding. The AT-rich domain of the OsCslF6 promoter varies in rice varieties from different locations and natural variants in this domain were associated with BPH resistance. MLG-derived oligosaccharides bound to the plasma membrane-anchored LECTIN RECEPTOR KINASE1 OsLecRK1 and modulated its activity. Thus, our findings suggest that the OsMYC2-OsCslF6 module regulates pest resistance by modulating MLG production to enhance vascular wall thickness and OsLecRK1-mediated defence signalling during rice-BPH interactions.

中文翻译:

禾本科特异性 β-1,3;1,4-d-葡聚糖在稻褐飞虱相互作用过程中将茉莉酸信号与 OsLecRK1 介导的防御反应联系起来

褐飞虱(BPH,Nilaparvata lugens)是一种极具破坏性的害虫,对全球水稻( Oryza sativa )生产构成严重威胁。茉莉酸是调节植物防御良性飞虱的关键植物激素;然而,茉莉酸与水稻中褐飞虱反应之间的分子联系仍然很大程度上未知。在这里,我们发现了一种禾本科特有的代谢产物,混合连接β-1,3;1,4- d-葡聚糖(MLG),它有助于茉莉酸介导的BPH抗性。稻米中的 MLG 水平在 BPH 攻击后显着增加。过表达OsCslF6(编码催化MLG生物合成的葡聚糖合酶)显着增强了BPH抵抗力和维管束中的细胞壁厚度,而OsCslF6的敲除降低了BPH抵抗力和血管壁厚度。OsMYC2 是茉莉酸信号转导的主要转录因子,直接控制OsCslF6响应 BPH 摄食而上调。OsCslF6启动子的富含 AT 的结构域在不同地点的水稻品种中有所不同,并且该结构域的天然变异与 BPH 抗性相关。MLG 衍生的寡糖与质膜锚定的凝集素受体激酶 1 OsLecRK1 结合并调节其活性。因此,我们的研究结果表明,OsMYC2-OsCslF6 模块通过调节 MLG 的产生来调节害虫抗性,从而增强水稻与 BPH 相互作用过程中的血管壁厚度和 OsLecRK1 介导的防御信号。
更新日期:2023-03-23
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