The inhibition of tyrosinase (TYR) activity is an effective measure to inhibit melanin synthesis. At present, there are many methods with discrepant details that study the TYR inhibitory activity of samples. Under the same experimental conditions, this paper systematically studies whether enzyme species and sample addition methods are the key factors that determine the TYR inhibitory activity of samples. TYRs extracted from B16F10 cells, apple and mushroom, called BTYR, ATYR and MTYR, respectively, were selected to implement this study. Results showed that TYR inhibitory activities of samples were obviously affected by the above two factors. It was necessary to select the appropriate enzyme according to the problems to be explained. It was speculated that indirectly inhibitory activity reflected the comprehensive effects of samples on TYR catalytic activity and intracellular TYR synthesis pathway, while directly inhibitory activity reflected the effects of samples on TYR catalytic activity. Additionally, kojic acid could be used as a positive control for both B16F10 cells and MTYR models. The TYR inhibitory activity of β-arbutin was complicated and fickle, while that of epigallocatechin gallate (EGCG) was universal and stable, which is to say, EGCG always inhibited TYR activity in a dose-dependent manner. In conclusion, the TYR inhibitory activities of samples were affected by enzyme species and sample addition methods. Compared with the unstable β-arbutin, EGCG was more valuable for clinical research.

中文翻译：

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样品对酪氨酸酶的抑制活性受酶种类和加样方法的影响

抑制酪氨酸酶（TYR）活性是抑制黑色素合成的有效措施。目前，研究样品对TYR抑制活性的方法有很多，细节也不尽相同。在相同的实验条件下，本文系统地研究了酶种类和加样方法是否是决定样品TYR抑制活性的关键因素。选择从 B16F10 细胞、苹果和蘑菇中提取的 TYR，分别称为 BTYR、ATYR 和 MTYR，以实施该研究。结果表明，样品的TYR抑制活性明显受上述两个因素的影响。有必要根据要解释的问题选择合适的酶。推测间接抑制活性反映了样品对TYR催化活性和细胞内TYR合成途径的综合影响，而直接抑制活性反映了样品对TYR催化活性的影响。此外，曲酸可用作 B16F10 细胞和 MTYR 模型的阳性对照。β-熊果苷的TYR抑制作用复杂多变，而表没食子儿茶素没食子酸酯(EGCG)的抑制作用普遍且稳定，即EGCG始终以剂量依赖的方式抑制TYR活性。总之，样品的 TYR 抑制活性受酶种类和样品添加方法的影响。与不稳定的β-熊果苷相比，EGCG更具有临床研究价值。而直接抑制活性反映了样品对TYR催化活性的影响。此外，曲酸可用作 B16F10 细胞和 MTYR 模型的阳性对照。β-熊果苷的TYR抑制作用复杂多变，而表没食子儿茶素没食子酸酯(EGCG)的抑制作用普遍且稳定，即EGCG始终以剂量依赖的方式抑制TYR活性。总之，样品的 TYR 抑制活性受酶种类和样品添加方法的影响。与不稳定的β-熊果苷相比，EGCG更具有临床研究价值。而直接抑制活性反映了样品对TYR催化活性的影响。此外，曲酸可用作 B16F10 细胞和 MTYR 模型的阳性对照。β-熊果苷的TYR抑制作用复杂多变，而表没食子儿茶素没食子酸酯(EGCG)的抑制作用普遍且稳定，即EGCG始终以剂量依赖的方式抑制TYR活性。总之，样品的 TYR 抑制活性受酶种类和样品添加方法的影响。与不稳定的β-熊果苷相比，EGCG更具有临床研究价值。而表没食子儿茶素没食子酸酯（EGCG）的抑制作用普遍且稳定，也就是说，EGCG总是以剂量依赖的方式抑制TYR的活性。总之，样品的 TYR 抑制活性受酶种类和样品添加方法的影响。与不稳定的β-熊果苷相比，EGCG更具有临床研究价值。而表没食子儿茶素没食子酸酯（EGCG）的抑制作用普遍且稳定，也就是说，EGCG总是以剂量依赖的方式抑制TYR的活性。总之，样品的 TYR 抑制活性受酶种类和样品添加方法的影响。与不稳定的β-熊果苷相比，EGCG更具有临床研究价值。