Metal ions and pesticides are extensively used in many industries and agriculture. However, they cause significant environmental pollution and various adverse health effects. Therefore, the development of sensitive and selective techniques to detect them is necessary for human health and the ecosystem. In this paper, we report a novel red-emitting fluorescence probe with a large Stokes shift (∼220 nm) based on rhodamine and isophorone units. The probe shows a ratiometric fluorescence response toward Hg²⁺ ions; however, Cu²⁺ ions quench the red fluorescence signal. The decomposition of the probe-Cu²⁺ complex allows detection of Thiram followed by recovery of the red fluorescence signal of the probe. In addition, the probe shows a good linear response to Hg²⁺, Cu²⁺, and Thiram, with detection limits of 122.0 nM, 29.0 nM, and 72.0 nM, respectively. The practical applicability of the probe has been successfully tested in real samples. Moreover, smartphone detection and light-responsive capsule fabrication have been established, for easy and quick detection. The probe possesses very low cytotoxicity and allows visualization of Hg²⁺ and Cu²⁺ ions in HeLa cells. Therefore, the present probe is expected to be an effective tool assisting in easy, quick, and reliable detection of Thiram, Hg²⁺, and Cu²⁺ ions.

金属离子和杀虫剂广泛用于许多工业和农业。然而，它们会造成严重的环境污染和各种不利的健康影响。因此，开发灵敏和选择性的技术来检测它们对于人类健康和生态系统是必要的。在本文中，我们报告了一种基于罗丹明和异佛尔酮单元的具有大斯托克斯位移 (~220 nm) 的新型红色荧光探针。^{该探针显示出对 Hg 2+}离子的比率荧光响应；然而，Cu ²⁺离子淬灭红色荧光信号。^{探针-Cu 2+}复合物的分解允许检测福美双，然后恢复探针的红色荧光信号。此外，探头对 Hg 显示出良好的线性响应²⁺、Cu ²⁺和 Thiram，检测限分别为 122.0 nM、29.0 nM 和 72.0 nM。该探针的实际适用性已在实际样品中得到成功验证。此外，已经建立了智能手机检测和光敏胶囊制造，以便于快速检测。该探针具有极低的细胞毒性，可显示HeLa 细胞中的Hg ²⁺和 Cu ^2+离子。因此，本探针有望成为一种有效的工具，有助于轻松、快速、可靠地检测福美双、Hg ²⁺和Cu ²⁺离子。