The potential risk of insecticidal proteins produced by genetically engineered (GE) plants to nontarget organisms have long been an ecotoxicological concern. Apanteles chilonis, an important endoparasitoid of rice pest Chilo suppressalis, potentially is exposed to Bacillus thuringiensis (Bt) endotoxins through a food chain of transgenic Bt rice – C. suppressalis – A. chilonis, and thus, a rigorous risk assessment is urgently needed. Here, we combined a tri-trophic bioassay system with high-dose exposure approach using C. suppressalis hemolymph as the carrier of insecticidal protein to evaluate the biosafety of Cry1Ca to A. chilonis.

中文翻译：

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从基因工程植物到内寄生生物的食物链转移过程中监测 Cry1Ca 积累减少和风险评估

基因工程（GE）植物产生的杀虫蛋白对非目标生物体的潜在风险长期以来一直是生态毒理学关注的问题。二化螟是水稻害虫二化螟重要的内寄生蜂，其可能通过转基因Bt水稻-二化螟-二化螟的食物链接触苏云金芽孢杆菌( Bt )内毒素，因此迫切需要进行严格的风险评估。在这里，我们将三营养生物测定系统与高剂量暴露方法相结合，以二化螟血淋巴作为杀虫蛋白载体，评估Cry1Ca对螟螟的生物安全性。