Corn oil, sodium carboxymethyl cellulose (CMC-Na), and dimethyl sulfoxide (DMSO) are widely used as solvents or suspensions in animal experiments, but the effects of prenatal exposure to them on fetal development have not been reported. In this study, Kunming mice were given a conventional dose of corn oil (9.2 g/kg·d), CMC-Na (0.05 g/kg·d) or DMSO (0.088 g/kg·d) during gestation days 10–18, and the pregnancy outcome, fetal physical development, serum phenotype, and multi-organ function changes were observed. The results showed that corn oil decreased serum triglyceride level in males but increased their serum testosterone and CORT levels, and affected female placenta and female/male multi-organ functions (mainly bone, liver, kidney). CMC-Na increased female/male body lengths and tail lengths, decreased serum glucose and total cholesterol levels in males as well as increased their serum LDL-C/HDL-C ratio and testosterone level, decreased female serum bile acid level, and affected male/female placenta and multi-organ functions (mainly bone, liver, hippocampus). DMSO decreased male body weight and serum glucose level, decreased male/female serum bile acid levels, and affected male/female placenta and multi-organs functions (mainly bone, hippocampus, adrenal gland). In conclusion, prenatal exposure to a conventional dose of corn oil, CMC-Na or DMSO could affect fetal physical development and multi-organ functions, and has the characteristics of "multi-pathway, multi-organ and multi-target". This study provides the experimental basis for the rational selection of solvents or suspensions in pharmacology and toxicology studies.

玉米油、羧甲基纤维素钠（CMC-Na）和二甲基亚砜（DMSO）在动物实验中被广泛用作溶剂或悬浮液，但产前接触它们对胎儿发育的影响尚未见报道。在本研究中，昆明小鼠在妊娠第 10-18 天给予常规剂量的玉米油（9.2 g/kg·d）、CMC-Na（0.05 g/kg·d）或 DMSO（0.088 g/kg·d） ,观察妊娠结局、胎儿体格发育、血清表型及多脏器功能变化。结果表明，玉米油降低了男性血清甘油三酯水平，但增加了他们的血清睾酮和CORT水平，并影响了女性胎盘和女性/男性多器官功能（主要是骨、肝、肾）。CMC-Na 增加雌性/雄性体长和尾长，降低男性血糖和总胆固醇水平，增加血清低密度脂蛋白胆固醇/高密度脂蛋白胆固醇比值和睾酮水平，降低女性血清胆汁酸水平，影响男性/女性胎盘和多器官功能（主要是骨骼、肝脏） , 海马）。DMSO降低男性体重和血糖水平，降低男性/女性血清胆汁酸水平，影响男性/女性胎盘和多器官功能（主要是骨骼、海马、肾上腺）。综上所述，产前常规剂量玉米油、CMC-Na或DMSO暴露可影响胎儿身体发育和多器官功能，具有“多途径、多器官、多靶点”的特点。本研究为药理毒理研究中溶剂或混悬剂的合理选择提供了实验依据。