In situ bioremediation using organohalide-respiring bacteria (OHRB) is a prospective method for the removal of persistent halogenated organic pollutants from groundwater, as OHRB can utilize H₂ or organic compounds produced by carbon source materials as electron donors for cell growth through organohalide respiration. However, few previous studies have determined the suitability of different carbon source materials to the metabolic mechanism of reductive dehalogenation from the perspective of electron transfer. The focus of this critical review was to reveal the interactions and relationships between carbon source materials and functional microbes, in terms of the electron transfer mechanism. Furthermore, this review illustrates some innovative strategies that have used the physiological characteristics of OHRB to guide the optimization of carbon source materials, improving the abundance of indigenous dehalogenated bacteria and enhancing electron transfer efficiency. Finally, it is proposed that future research should combine multi-omics analysis with machine learning (ML) to guide the design of effective carbon source materials and optimize current dehalogenation bioremediation strategies to reduce the cost and footprint of practical groundwater bioremediation applications.

使用有机卤化物呼吸细菌 (OHRB) 进行原位生物修复是一种从地下水中去除持久性卤化有机污染物的前瞻性方法，因为 OHRB 可以利用 H ₂或由碳源材料产生的有机化合物作为电子供体，通过有机卤化物呼吸进行细胞生长。然而，以往很少有研究从电子转移的角度确定不同碳源材料对还原脱卤代谢机制的适应性。这篇批判性综述的重点是揭示碳源材料与功能微生物之间在电子转移机制方面的相互作用和关系。此外，这篇综述阐述了一些利用 OHRB 的生理特性来指导碳源材料优化、提高本土脱卤细菌丰度和提高电子转移效率的创新策略。最后，