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Logic-Gates of Gas Pressure for Portable, Intelligent and Multiple Analysis of Metal Ions
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-20 , DOI: 10.1021/acs.analchem.2c05677 Lu Shi 1 , Qiaorong Tang 1 , Bing Yang 1 , Wei Liu 1 , Baoxin Li 1 , Chaoyong Yang 2 , Yan Jin 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-20 , DOI: 10.1021/acs.analchem.2c05677 Lu Shi 1 , Qiaorong Tang 1 , Bing Yang 1 , Wei Liu 1 , Baoxin Li 1 , Chaoyong Yang 2 , Yan Jin 1
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DNA logic gates have shown outstanding magic in intelligent biology applications, but it remains challenging to construct a portable, affordable and convenient DNA logic gate. Herein, logic gates of gas pressure were innovatively developed for multiplex analysis of metal ions. Hg2+ and Ag+ were input to interact specifically with the respective mismatched base pairs, which activated DNA extension reaction by polymerase and led to the enrichment of platinum nanoparticles for catalyzing the decomposition of peroxide hydrogen. Thus, the gas pressure obtained from a sealed well was used as output for detecting or identifying metal ions. Hg2+ and Ag+ were sensitively and selectively detected, and the assay of the real samples was also satisfactory. Based on this, DNA logic gates, including YES, NOT, AND, OR, NAND, NOR, INHIBIT, and XOR were successfully established using a portable and hand-held gas pressure meter as detector. So, the interactions between DNA and metal ions were intelligently transferred into the output of gas pressure, which made metal ions to be detected portably and identified intelligently. Given the remarkable merits of simplicity, logic operation, and portable output, the metal ion-driven DNA logic gate of gas pressure provides a promising way for intelligent and portable biosensing.
中文翻译：
用于金属离子便携式、智能化和多重分析的气压逻辑门
DNA逻辑门在智能生物学应用中展现出非凡的魔力，但构建便携、经济、方便的DNA逻辑门仍然具有挑战性。在此，创新性地开发了气压逻辑门，用于金属离子的多重分析。输入Hg 2+和Ag +与各自错配的碱基对特异性相互作用，通过聚合酶激活DNA延伸反应，导致铂纳米粒子富集，催化过氧化氢分解。因此，从密封井中获得的气压被用作检测或识别金属离子的输出。Hg 2+和Ag +被灵敏和选择性地检测到，并且实际样品的测定也令人满意。在此基础上，使用便携式手持气体压力计作为检测器，成功建立了DNA逻辑门，包括YES、NOT、AND、OR、NAND、NOR、INHIBIT和XOR。于是，将DNA与金属离子的相互作用智能化转化为气体压力的输出，实现了金属离子的便携检测和智能识别。鉴于简单、逻辑运算和便携式输出的显着优点，金属离子驱动的气体压力 DNA 逻辑门为智能和便携式生物传感提供了一种有前途的方法。
更新日期：2023-03-20
中文翻译：
用于金属离子便携式、智能化和多重分析的气压逻辑门
DNA逻辑门在智能生物学应用中展现出非凡的魔力，但构建便携、经济、方便的DNA逻辑门仍然具有挑战性。在此，创新性地开发了气压逻辑门，用于金属离子的多重分析。输入Hg 2+和Ag +与各自错配的碱基对特异性相互作用，通过聚合酶激活DNA延伸反应，导致铂纳米粒子富集，催化过氧化氢分解。因此，从密封井中获得的气压被用作检测或识别金属离子的输出。Hg 2+和Ag +被灵敏和选择性地检测到，并且实际样品的测定也令人满意。在此基础上，使用便携式手持气体压力计作为检测器，成功建立了DNA逻辑门，包括YES、NOT、AND、OR、NAND、NOR、INHIBIT和XOR。于是，将DNA与金属离子的相互作用智能化转化为气体压力的输出，实现了金属离子的便携检测和智能识别。鉴于简单、逻辑运算和便携式输出的显着优点，金属离子驱动的气体压力 DNA 逻辑门为智能和便携式生物传感提供了一种有前途的方法。
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