Nowadays the multifunctional approaches to kill oral bacteria based on various nanocomposites have made great progress against periodontal infections, while the material structure and its functional integration are still insufficient. Herein, this work proposes a therapeutic strategy of chemodynamical therapy (CDT) and photothermal therapy (PTT) in monocrystals to effectively enhance the synergistic treatment. The CuS/MnS@MnO₂ consisting of hexagonal CuS/MnS nano-twin-crystal with a shell layer of MnO₂ is developed. In this nanosystem, the purpose of synergistic treatment of periodontitis by combining PTT/CDT is achieved within a CuS/MnS monocrystal, where CuS serves to achieve photothermal conversion, dissipate the biofilm and transfer the heat in situ to the integrated MnS, thus promoting the Mn²⁺-mediated CDT process. Meanwhile, the CDT process can generate the highly toxic hydroxyl radical to destroy extracellular DNA by utilization of endogenous H₂O₂ produced by Streptococci in the oral biofilm, cooperating with PTT to dissipate the bacterial biofilm. With the design of the outer shell of MnO₂, the selective bacteria-killing can be realized by producing oxygen which can protect the periodontal non-pathogenic aerobic bacteria and threaten the survival of anaerobic pathogens. Therefore, such design via multipattern strategies to combat microorganisms would provide a bright prospect for the clinical treatment of bacterial infections.

中文翻译：

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MnO2 修饰的新型双晶纳米片通过多模式策略对抗细菌生物膜以抵抗牙周感染

目前，基于各种纳米复合材料的多功能杀灭口腔细菌的方法在对抗牙周感染方面取得了很大进展，但材料结构及其功能集成仍然不足。在此，这项工作提出了单晶化学动力学疗法（CDT）和光热疗法（PTT）的治疗策略，以有效增强协同治疗。开发了由六方CuS/MnS纳米孪晶和MnO ₂壳层组成的CuS/MnS @MnO _{2 。}在该纳米系统中，通过结合PTT/CDT协同治疗牙周炎的目的是在CuS/MnS单晶内实现的，其中CuS起到光热转换的作用，消散生物膜并将热量原位传递给集成的MnS，从而促进Mn ²⁺介导的CDT过程。同时，CDT过程利用口腔生物膜中链球菌产生的内源性H ₂ O _{2 ，​​产生剧毒的羟基自由基，破坏细胞外DNA，配合PTT消散细菌生物膜。通过MnO 2}外壳的设计，可以通过产生氧气来实现选择性杀菌，保护牙周非致病性需氧菌，威胁厌氧菌的生存。因此，这种通过多模式策略来对抗微生物的设计将为细菌感染的临床治疗提供光明的前景。