In this study, silane reagents were for the first time explored as the fluorescence sensitizer. They were demonstrated to have fluorescence sensitization effect on curcumin and 3-glycidoxypropyltrimethoxysilane (GPTMS) possessed the strongest effect. Thus, GPTMS was adopted as the novel fluorescence sensitizer to turn on the fluorescence of curcumin by more than two orders of magnitude for detection. In this way, curcumin could be determined with a linear range of 0.2–2000 ng/mL and an LOD of 0.067 ng/mL. The method was applicable to determine curcumin in several actual food samples, which had the good consistency with the high performance liquid chromatographic method, demonstrating the high accuracy of the proposed method. In addition, the curcumins sensitized by GPTMS could be cured under certain conditions and held the potential for solid fluorescence application. This study expanded the scope of fluorescence sensitizer to silane reagents, and provided the novel approach for fluorescence detection of curcumin and further to generate new solid fluorescence system.

在这项研究中，硅烷试剂首次被探索作为荧光敏化剂。它们被证明对姜黄素具有荧光增敏作用，其中 3-缩水甘油氧基丙基三甲氧基硅烷 (GPTMS) 具有最强的作用。因此，采用 GPTMS 作为新型荧光敏化剂，将姜黄素的荧光提高两个数量级以上进行检测。这样，姜黄素的线性范围为 0.2–2000 ng/mL，LOD 为 0.067 ng/mL。该方法适用于多个实际食品样品中姜黄素的测定，与高效液相色谱法具有良好的一致性，表明该方法准确度高。此外，GPTMS敏化的姜黄素在一定条件下可以固化，具有固体荧光应用的潜力。本研究将荧光敏化剂的范围扩大到硅烷试剂，为姜黄素的荧光检测提供了新的途径，进而生成新的固体荧光体系。