Plasmon-enhanced luminescence (PEL) is a unique photophysical phenomenon in which the interaction between luminescent moieties and metal nanostructures results in a marked luminescence enhancement. PEL offers several advantages and has been extensively used to design robust biosensing platforms for luminescence-based detection and diagnostics applications, as well as for the development of many efficient bioimaging platforms, enabling high-contrast non-invasive real-time optical imaging of biological tissues, cells, and organelles with high spatial and temporal resolution. This review summarizes recent progress in the development of various PEL-based biosensors and bioimaging platforms for diverse biological and biomedical applications. Specifically, we comprehensively assessed rationally designed PEL-based biosensors that can efficiently detect biomarkers (proteins and nucleic acids) in point-of-care tests, highlighting significant improvements in the sensing performance upon the integration of PEL. In addition to discussing the merits and demerits of recently developed PEL-based biosensors on substrates or in solutions, we include a brief discussion on integrating PEL-based biosensing platforms into microfluidic devices as a promising multi-responsive detection method. The review also presents comprehensive details about the recent advances in the development of various PEL-based multi-functional (passive targeting, active targeting, and stimuli-responsive) bioimaging probes, highlighting the scope of future improvements in devising robust PEL-based nanosystems to achieve more effective diagnostic and therapeutic insights by enabling imaging-guided therapy.

等离子体增强发光 (PEL) 是一种独特的光物理现象，其中发光部分和金属纳米结构之间的相互作用导致显着的发光增强。PEL 具有多种优势，已广泛用于为基于发光的检测和诊断应用设计稳健的生物传感平台，以及开发许多高效的生物成像平台，实现生物组织的高对比度非侵入性实时光学成像、细胞和细胞器具有高空间和时间分辨率。这篇综述总结了为各种生物和生物医学应用开发各种基于 PEL 的生物传感器和生物成像平台的最新进展。具体来说，我们全面评估了合理设计的基于 PEL 的生物传感器，这些传感器可以在即时检验中有效检测生物标志物（蛋白质和核酸），强调了集成 PEL 后传感性能的显着改进。除了讨论最近开发的基于 PEL 的生物传感器在基板上或溶液中的优缺点外，我们还简要讨论了将基于 PEL 的生物传感平台集成到微流体设备中作为一种有前途的多响应检测方法。该综述还全面详细介绍了各种基于 PEL 的多功能（被动靶向、主动靶向和刺激响应）生物成像探针的最新进展，