Little attention has been paid to the in situ-generated biogenic manganese oxides (BMnOx) for practical implementation in continuous groundwater remediation systems. The enrichment effects of manganese oxidizing bacteria (MOB) in bioaugmentation columns and the in situ-generated BMnOx for continuous thallium(I) (Tl(I)) removal from groundwater were investigated. Results indicated that Pseudomonas Putida MnB1 (strain MnB1) attached on the groundwater sediments (GS) can achieve a maximum of 97.37 % Mn(II) oxidation and generate 29.6 mg/L BMnOx, which was superior than that of traditional quartz sand (QS). The in situ-generated BMnOx in MOB_GS column effectively removed 10–100 μg/L Tl(I) under the interference of high concentrations of Fe(II) and Mn(II) in groundwater. Distinctive microbial enrichment effects occurred in the bioaugmentation columns under the competition of indigenous microbes in groundwater. The release of Mn(II) from the BMnOx inhibited with the decrease in Tl(I) removal efficiency. XAFS analysis revealed Tl(I) was effectively adsorbed by BMnOx and Mn-O octahedra with Tl-O tetrahedral coordination existed in BMnOx. This study provides an in-depth understanding of the in situ-generated BMnOx for the Tl(I) removal and contributes to the application of BMnOx in groundwater remediation.

很少有人关注原位生成的生物锰氧化物 (BMnOx) 在连续地下水修复系统中的实际应用。研究了锰氧化细菌 (MOB) 在生物强化柱中的富集效果和原位生成的 BMnOx 对地下水中连续铊 (I) (Tl(I)) 去除的影响。结果表明，附着在地下水沉积物 (GS) 上的恶臭假单胞菌MnB1（菌株 MnB1）最多可实现 97.37% 的 Mn(II) 氧化，并产生 29.6 mg/L BMnOx，优于传统石英砂 (QS) . 就地_-在地下水中高浓度 Fe(II) 和 Mn(II) 的干扰下，MOB_GS 柱中生成的 BMnOx 可有效去除 10–100 μg/L Tl(I)。在地下水中土著微生物的竞争下，生物强化柱中发生了独特的微生物富集效应。随着 Tl(I) 去除效率的降低，BMnOx 中 Mn(II) 的释放受到抑制。XAFS 分析表明 Tl(I) 被 BMnOx 有效吸附，BMnOx 中存在 Mn-O 八面体和 Tl-O 四面体配位。本研究深入了解原位生成的 BMnOx 以去除 Tl(I)，并有助于 BMnOx 在地下水修复中的应用。