Abnormal viscosity and excessive superoxide anion (O₂^•-) levels in living cells often cause a series of biological dysfunction and oxidative damage. However, a great challenge remains in quickly and conveniently detecting the viscosity and O₂^•- levels in living cells. Herein, we fabricated a versatile aggregation-induced emission (AIE) probe with mitochondria targeting, DTPB, for dual-imaging of viscosity and O₂^•- level in living cells with two different channels. The obtained DTPB contained a diphenyl phosphinic acid unit responsive to O₂^•-, a unit with twisted intramolecular charge trans (TICT) function responsive to viscosity, and a pyridine cation unit with mitochondria targeting. The results showed that DTPB exhibited a remarkable response to viscosity with a near-infrared emission peak at 671 nm and was highly sensitive to O₂^•- levels with an emission peak at 587 nm. The dual-channel probe has great application prospects in the visual diagnosis of cancer and related diseases.

活细胞黏度异常和超氧阴离子（O ₂ ^•-）水平过高，常引起一系列生物功能障碍和氧化损伤。然而，快速方便地检测活细胞的粘度和O ₂ ^{•-水平仍然是一个巨大的挑战。}在此，我们制作了一种具有线粒体靶向功能的多功能聚集诱导发射 (AIE) 探针 DTPB，用于在具有两个不同通道的活细胞中对粘度和 O 2 • _-^{水平进行双重成像。}获得的DTPB含有一个对O _{2敏感的二苯基次膦酸单元}^•-，一个具有对粘度敏感的扭曲分子内电荷反式（TICT）功能的单元，以及一个具有线粒体靶向功能的吡啶阳离子单元。结果表明，DTPB 对粘度有显着响应，在 671 nm 有近红外发射峰，对 O ₂ ^•-水平高度敏感，在 587 nm 有发射峰。双通道探头在癌症及相关疾病的视觉诊断中具有广阔的应用前景。