In insects, lipids and trehalose are the primary energy reserves and sources, and their metabolism is controlled by a variety of physiological processes. Adipokinetic hormone receptor (AKHR) has been reported to be involved in glycolipid metabolism and immune response, however, is an uncharted territory in Helicoverpa armigera. In the present study, the full-length cDNA encoding AKHR was isolated from H. armigera. The HaAKHR contains a seven-transmembrane domain, which was a characteristic of G-protein coupled receptors. The expression profile analysis revealed that HaAKHR was highly expressed in fat body of newly emerged female adults and highly expressed in the immature stage. The content of triglyceride (TAG) in fat body increased significantly after silencing of HaAKHR, however, the trehalase activity and the expression of trehalose-6-phosphate synthase (TPS) were decreased in hemolymph. Furthermore, the expression level of HaAKHR was significantly upregulated when infected with different kinds of entomopathogenic microbes. The above results suggested that HaAKHR plays an important role in regulating glycolipid metabolism and immune response in H. armigera, and contribute to understanding the underlying mechanism of host-pathogen interactions in H. armigera.

在昆虫中，脂质和海藻糖是主要的能量储备和来源，它们的代谢受多种生理过程控制。据报道，脂肪动力学激素受体 (AKHR) 参与糖脂代谢和免疫反应，但在棉铃虫中是一个未知领域。在本研究中，从棉铃虫中分离出编码 AKHR 的全长 cDNA 。HaAKHR 包含一个七次跨膜结构域，这是 G 蛋白偶联受体的一个特征。表达谱分析表明，HaAKHR在新生雌成虫的脂肪体中高表达，在未成熟期高表达。沉默HaAKHR后脂肪体中甘油三酯（TAG）含量显着增加，而血淋巴中海藻糖酶活性和海藻糖-6-磷酸合酶（TPS ）表达降低。此外，当感染不同种类的昆虫病原微生物时， HaAKHR的表达水平显着上调。上述结果表明，HaAKHR在调控棉铃虫糖脂代谢和免疫应答方面发挥着重要作用，有助于理解棉铃虫宿主-病原体相互作用的潜在机制。棉铃虫。