Chronic kidney disease (CKD) is a disease with decreased, irreversible renal function. Pruritus is the most common skin symptom in patients with CKD, especially in end-stage renal disease. The underlying molecular and neural mechanism of CKD-associated pruritus (CKD-aP) remains obscure. Our data show that the level of allantoin increases in the serum of CKD-aP and CKD model mice. Allantoin could induce scratching behavior in mice and active DRG neurons. The calcium influx and action potential reduced significantly in DRG neurons of MrgprD KO or TRPV1 KO mice. U73122, an antagonist of phospholipase C, could also block calcium influx in DRG neurons induced by allantoin. Thus, our results concluded that allantoin plays an important role in CKD-aP, mediated by MrgprD and TrpV1, in CKD patients.

中文翻译：

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尿囊素通过激活慢性肾脏病中的 MrgprD 诱发瘙痒

慢性肾脏病 (CKD) 是一种肾功能下降且不可逆的疾病。瘙痒是 CKD 患者最常见的皮肤症状，尤其是终末期肾病患者。CKD 相关瘙痒症 (CKD-aP) 的潜在分子和神经机制仍然不清楚。我们的数据显示尿囊素水平在 CKD-aP 和 CKD 模型小鼠的血清中增加。尿囊素可以诱导小鼠和活跃的 DRG 神经元的抓挠行为。MrgprD KO 或 TRPV1 KO 小鼠的 DRG 神经元的钙内流和动作电位显着降低。U73122 是磷脂酶 C 的拮抗剂，也可以阻断尿囊素诱导的 DRG 神经元中的钙流入。因此，我们的结果得出结论，尿囊素在 CKD 患者的 CKD-aP 中起重要作用，由 MrgprD 和 TrpV1 介导。