Two Antenna-Enriched Carboxylesterases Mediate Olfactory Responses and Degradation of Ester Volatiles in the German Cockroach Blattella germanica,Journal of Agricultural and Food Chemistry

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Two Antenna-Enriched Carboxylesterases Mediate Olfactory Responses and Degradation of Ester Volatiles in the German Cockroach Blattella germanica
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-03-15 , DOI: 10.1021/acs.jafc.2c08488
Yun-Feng Ma ₁ , Lang-Lang Gong ₁ , Meng-Qi Zhang ₁ , Xuan-Zheng Liu ₁ , Huan Guo ₁ , J Joe Hull ₂ , Gui-Jun Long ₁ , Hong Wang ₁ , Youssef Dewer ₃ , Fan Zhang ₄ , Ming He ₁ , Peng He ₁

Affiliation

Insects have evolved an extremely sensitive olfactory system that is essential for a series of physiological and behavioral activities. Some carboxylesterases (CCEs) comprise a major subfamily of odorant-degrading enzymes (ODEs) playing a crucial role in odorant signal inactivation to maintain the odorant receptor sensitivity. In this study, 93 CCEs were annotated in the genome of the German cockroach Blattella germanica, a serious urban pest. Phylogenetic and digital tissue expression pattern analyses identified two antenna-enriched CCEs, BgerCCE021e3 and BgerCCE021d1, as candidate ODEs. RNA interference (RNAi)-mediated knockdown of BgerCCE021e3 and BgerCCE021d1 resulted in partial anosmia with experimental insects exhibiting reduced attraction to ester volatile resources and slower olfactory responses than controls. Furthermore, enzymatic conversion of geranyl acetate by crude male antennal extracts from BgerCCE021e3 and BgerCCE021d1 RNAi insects was also significantly reduced. Our results provide evidence for CCE function in German cockroach olfaction and provide a basis for further exploring behavioral inhibitors that target olfactory-related CCEs.

中文翻译：

两种富含天线的羧酸酯酶介导德国小蠊德国小蠊的嗅觉反应和酯类挥发物的降解

昆虫已经进化出一种极其敏感的嗅觉系统，这对一系列生理和行为活动至关重要。一些羧酸酯酶 (CCE) 包含一个主要的气味降解酶 (ODE) 亚科，在气味信号失活以维持气味受体敏感性方面起着至关重要的作用。在这项研究中，在德国小蠊Blattella germanica的基因组中注释了 93 个 CCE ，这是一种严重的城市害虫。系统发育和数字组织表达模式分析确定了两个富含天线的 CCE，BgerCCE021e3和BgerCCE021d1，作为候选 ODE。RNA 干扰 (RNAi) 介导的BgerCCE021e3和BgerCCE021d1敲低导致部分嗅觉丧失，实验昆虫表现出对酯类挥发性资源的吸引力降低，嗅觉反应比对照组慢。此外，来自BgerCCE021e3和BgerCCE021d1 RNAi 昆虫的粗雄性触角提取物对乙酸香叶酯的酶促转化也显着减少。我们的结果为德国小蠊嗅觉中的 CCE 功能提供了证据，并为进一步探索针对嗅觉相关 CCE 的行为抑制剂提供了基础。

更新日期：2023-03-15

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